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Mouse Anti-BHMT Recombinant Antibody (CBYY-0547) (CBMAB-0550-YY)


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Published Data
Mouse Anti-BHMT Recombinant Antibody (CBYY-0547) (CBMAB-0550-YY) in IHC
BHMT is present in a human MS lesion. (A) Postmortem MS tissue stained for PLP. A white matter lesion is evident. Area shown in (B) is from boxed white matter lesion area on adjacent section. Brightfield images taken at 40x show colocalization of OLIG2 (blue alkaline phosphatase staining) and BHMT immunoreactivity (brown DAB precipitate) denoted by arrows on cortical tissue sections. ...View More
Sternbach, S., West, N., Singhal, N. K., Clements, R., Basu, S., Tripathi, A., ... & McDonough, J. (2021). The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturation. PloS one, 16(5), e0250486.
Mouse Anti-BHMT Recombinant Antibody (CBYY-0547) (CBMAB-0550-YY) in WB and IP
(A) Representative Western blot images for BHMT, DNMT3a, and H3K4me3 from nuclear chromatin fractions of MO3.13 oligodendrocytes treated with 400 μM SNP. Lanes are denoted "C" for control and "S" for SNP treated. n = 3. (B) Co-IP analysis of protein-protein interactions between BHMT and DNMT3a in MO3.13 oligodendrocytes. ...View More
Sternbach, S., West, N., Singhal, N. K., Clements, R., Basu, S., Tripathi, A., ... & McDonough, J. (2021). The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturation. PloS one, 16(5), e0250486.
Mouse Anti-BHMT Recombinant Antibody (CBYY-0547) (CBMAB-0550-YY) in IF
Confocal image shows BHMT+ (green fluorescence) and NeuN+ neurons (red fluorescence) in the mouse hippocampus. ...View More
Singhal, N. K., Sternbach, S., Fleming, S., Alkhayer, K., Shelestak, J., Popescu, D., ... & McDonough, J. (2020). Betaine restores epigenetic control and supports neuronal mitochondria in the cuprizone mouse model of multiple sclerosis. Epigenetics, 15(8), 871-886.
Mouse Anti-BHMT Recombinant Antibody (CBYY-0547) (CBMAB-0550-YY) in WB
Chromatin fractionation of chromatin isolated from cultured neurons shows that BHMT is in the nucleus and is present in tight chromatin fractions (1.2 M, 1.8 M NaCl fractions). ...View More
Singhal, N. K., Sternbach, S., Fleming, S., Alkhayer, K., Shelestak, J., Popescu, D., ... & McDonough, J. (2020). Betaine restores epigenetic control and supports neuronal mitochondria in the cuprizone mouse model of multiple sclerosis. Epigenetics, 15(8), 871-886.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-0547
Antibody Isotype
IgG2a
Application
ELISA, WB, IP

Basic Information

Immunogen
Full length recombinant BHMT of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Betaine--Homocysteine S-Methyltransferase
Introduction
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Entrez Gene ID
635
UniProt ID
Q93088
Alternative Names
Betaine--Homocysteine S-Methyltransferase; EC 2.1.1.5; Epididymis Secretory Sperm Binding Protein Li 61p; Betaine--Homocysteine S-Methyltransferase 1; Betaine Homocysteine Methyltransferase; HEL-S-61p; EC 2.1.1; BHMT1;
Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Biological Process
Amino-acid betaine catabolic process Source: UniProtKB-UniPathway
Amino-acid betaine metabolic process Source: BHF-UCL
Choline catabolic process Source: Reactome
L-methionine salvage Source: BHF-UCL
Protein methylation Source: UniProtKB
Regulation of homocysteine metabolic process Source: UniProtKB
Sulfur amino acid metabolic process Source: Reactome
Cellular Location
Cytoplasm
More Infomation

Sternbach, S., West, N., Singhal, N. K., Clements, R., Basu, S., Tripathi, A., ... & McDonough, J. (2021). The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturation. PloS one, 16(5), e0250486.

Na, J. D., Choi, Y. J., Jun, D. S., & Kim, Y. C. (2019). Alleviation of paraquat-induced oxidative lung injury by betaine via regulation of sulfur-containing amino acid metabolism despite the lack of betaine-homocysteine methyltransferase (BHMT) in the lung. Food & function, 10(2), 1225-1234.

Maclean, K. N., Jiang, H., Phinney, W. N., Keating, A. K., Hurt, K. J., & Stabler, S. P. (2019). Taurine alleviates repression of betaine‐homocysteine S‐methyltransferase and significantly improves the efficacy of long‐term betaine treatment in a mouse model of cystathionine β‐synthase–deficient homocystinuria. The FASEB Journal, 33(5), 6339-6353.

Saande, C. J., Pritchard, S. K., Worrall, D. M., Snavely, S. E., Nass, C. A., Neuman, J. C., ... & Schalinske, K. L. (2019). Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats. The Journal of nutrition, 149(8), 1369-1376.

Partearroyo, T., Murillo‐Cuesta, S., Vallecillo, N., Bermúdez‐Muñoz, J. M., Rodríguez‐de LaRosa, L., Mandruzzato, G., ... & Varela‐Nieto, I. (2019). Betaine‐homocysteine S‐methyltransferase deficiency causes increased susceptibility to noise‐induced hearing loss associated with plasma hyperhomocysteinemia. The FASEB Journal, 33(5), 5942-5956.

Wu, L., Zhou, X., Li, T., He, J., Huang, L., Ouyang, Z., ... & He, Q. (2018). Improved Sp1 and betaine homocysteine-S-methyltransferase expression and homocysteine clearance are involved in the effects of zinc on oxidative stress in high-fat-diet-pretreated mice. Biological trace element research, 184(2), 436-441.

Garrido, F., Pacheco, M., Vargas-Martínez, R., Velasco-García, R., Jorge, I., Serrano, H., ... & Pajares, M. Á. (2018). Identification of hepatic protein-protein interaction targets for betaine homocysteine S-methyltransferase. PloS one, 13(6), e0199472.

Prieur, E. A., Pjetri, E., Zeisel, S. H., & Jadavji, N. M. (2017). Reduced brain volume and impaired memory in betaine homocysteine S-methyltransferase knockout mice. Applied Physiology, Nutrition, and Metabolism, 42(11), 1228-1231.

Pérez-Miguelsanz, J., Vallecillo, N., Garrido, F., Reytor, E., Pérez-Sala, D., & Pajares, M. A. (2017). Betaine homocysteine S-methyltransferase emerges as a new player of the nuclear methionine cycle. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1864(7), 1165-1182.

Mostashari-Mohases, M., Sadeghi, A. A., Ahmadi, J., & Esmaeilkhanian, S. (2017). Effect of Betaine Supplementation on Performance Parameters, Betaine-homocysteine S-methyltransferase Gene Expression in Broiler Chickens Consume Drinking Water with Different Total Dissolved Solids. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(4).

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For research use only. Not intended for any clinical use.

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