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Mouse Anti-ARID3A Antibody (A4) (CBMAB-0128-YC)


See all ARID3A antibodies
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Published Data
Mouse Anti-ARID3A Antibody (A4) CBMAB-0128-YC in IF
Immunofluorescence assays showed NF-κB p65 affecting nuclear import of ARID3A. ...View More
You, X., Liu, M., Liu, Q., Li, H., Qu, Y., Gao, X., ... & Xu, D. (2022). miRNA let-7 family regulated by NEAT1 and ARID3A/NF-κB inhibits PRRSV-2 replication in vitro and in vivo. PLoS Pathogens, 18(10), e1010820.
Mouse Anti-ARID3A Antibody (A4) CBMAB-0128-YC in IF
Triple immunofluorescence of murine B cells stained for actin (green), ARID3A (red), and nuclei (blue) revealed strong actin-ARID3A association. ...View More
Schmidt, C., Christian, L., Smith, T. A., Tidwell, J., Kim, D., & Tucker, H. O. (2021). Lipid Rafts Interaction of the ARID3A Transcription Factor with EZRIN and G-Actin Regulates B-Cell Receptor Signaling. Diseases, 9(1), 22.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A4
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 565-592 at the C-terminus of ARID3A of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ARID3A
Introduction
AT-rich interactive domain-containing protein 3A (ARID3A) is a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. ARID3A has been shown to interact with BTK and E2F1.
Entrez Gene ID
Human1820
Frog496519
UniProt ID
HumanQ99856
FrogQ6GQD7
Alternative Names
AT Rich Interactive Domain 3A (BRIGHT-Like); B-Cell Regulator Of IgH Transcription; ARID Domain-Containing Protein 3A; Dead Ringer-Like Protein 1; E2F-Binding Protein 1; BRIGHT; E2FBP1; DRIL1; DRIL3; AT Rich Interactive Domain 3A (BRIGHT- Like) Protein; AT Rich Interactive Domain 3A (BRIGHT- Like); Dead Ringer-Like 1 (Drosophila); ARID Domain-Containing 3A; Dead Ringer-Like 1; DRX
Function
Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
Biological Process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Cytoplasm; Nucleus. Shuttles between nucleus and cytoplasm.
More Infomation

Tang, J., Yang, L., Li, Y., Ning, X., Chaulagain, A., Wang, T., & Wang, D. (2021). ARID3A promotes the development of colorectal cancer by upregulating AURKA. Carcinogenesis, 42(4), 578-586.

Garton, J. W., Shankar, M., Guthridge, J., James, J. A., & Webb, C. (2021). ARID3a Defines a Novel Subset of Naïve B Cells in SLE Patients.

Arman, K., Saadat, K. A., Igci, Y. Z., Bozgeyik, E., Ikeda, M. A., Cakmak, E. A., & Arslan, A. (2020). Long noncoding RNA ERICD interacts with ARID3A via E2F1 and regulates migration and proliferation of osteosarcoma cells. Cell Biology International, 44(11), 2263-2274.

Ratliff, M. L., Shankar, M., Guthridge, J. M., James, J. A., & Webb, C. F. (2020). TLR engagement induces ARID3a in human blood hematopoietic progenitors and modulates IFNα production. Cellular immunology, 357, 104201.

Saadat, K. A., Bozgeyik, E., Arman, K., Bozgeyik, I., & Ikeda, M. A. (2020). ARID3A-mediated modulation of TP73 and TP73-AS1 in osteosarcoma cells. Gene Reports, 20, 100683.

Yoon, G., Park, J. Y., Kim, H. J., Choi, G. S., Kim, J. G., Kang, B. W., ... & Seo, A. N. (2019). ARID3A positivity correlated with favorable prognosis in patients with residual rectal cancer after Neoadjuvant chemoradiotherapy. Anticancer research, 39(6), 2845-2853.

Garton, J., Barron, M. D., Ratliff, M. L., & Webb, C. F. (2019). New Frontiers: ARID3a in SLE. Cells, 8(10), 1136.

Garton, J. W., Chapman, B., Shankar, M., Rose, K., & Webb, C. (2019). The DNA-binding protein ARID3a is Required for Human Erythrocyte Lineage Differentiation using the K562 Model Cell Line.

Garton, J. W., Pelikan, R., Ratliff, M., James, J. A., Gaffney, P., & Webb, C. F. (2018). Genes associated with ARID3a expression in B Lymphocytes from Systemic Lupus Erythematosus patients.

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For research use only. Not intended for any clinical use.

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