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Mouse Anti-ANTXR1 Recombinant Antibody (SB20) (CBMAB-A2868-YC)


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Published Data
Mouse Anti-ANTXR1 Recombinant Antibody (SB20) in FC
(A) Percentage (%) of the different clusters among CAF-S1 fibroblasts based on scRNA-seq data, each CAF-S1 cell being attributed as shown in Fig. 1A. (B) UMAP plots showing RNA levels of surface markers that are specific of each CAF-S1 cluster. (C) FACS plots from a representative BC patient showing the gating strategy used for validating the 5 most abundant CAF-S1 clusters shown in (B) by combining protein surface markers. ...View More
Kieffer, Y., Hocine, H. R., Gentric, G., Pelon, F., Bernard, C., Bourachot, B., ... & Mechta-Grigoriou, F. (2020). Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer. Cancer Discovery, 10(9), 1330-1351.
Mouse Anti-ANTXR1 Recombinant Antibody (SB20) in WB
TEM8 associates with the insoluble cytoskeleton independently of the presence of intermediatefilaments.Western blot analysis of TEM8, actin, tubulin, and vimentin distribution after Triton X-100 extraction of SW13 cells with (lanes 3 and 4) or without (lanes 1 and 2) vimentin intermediate filaments, infected for 24 h with HA-tagged TEM8 recombinant adenovirus (n=1). ...View More
Werner, E., Kowalczyk, A. P., & Faundez, V. (2006). Anthrax toxin receptor 1/tumor endothelium marker 8 mediates cell spreading by coupling extracellular ligands to the actin cytoskeleton. Journal of Biological Chemistry, 281(32), 23227-23236.
Mouse Anti-ANTXR1 Recombinant Antibody (SB20) in IHC and IF
Sections of umbilical cord were stained using TEM8 and/or VEGFR2-specific antibodies and visualized with DAB (A) or by immunofluorescence (B). ...View More
Hotchkiss, K. A., Basile, C. M., Spring, S. C., Bonuccelli, G., Lisanti, M. P., & Terman, B. I. (2005). TEM8 expression stimulates endothelial cell adhesion and migration by regulating cell-matrix interactions on collagen. Experimental cell research, 305(1), 133-144.
Mouse Anti-ANTXR1 Recombinant Antibody (SB20) in Immunoblot
TEM8 expression was probed by immunoblotting lysates of HUVEC from passages 1 to 3 with an antibody against the specific intracellular domain of TEM8 (SV1). TEM8 expression decreases with time in vitro. ...View More
Hotchkiss, K. A., Basile, C. M., Spring, S. C., Bonuccelli, G., Lisanti, M. P., & Terman, B. I. (2005). TEM8 expression stimulates endothelial cell adhesion and migration by regulating cell-matrix interactions on collagen. Experimental cell research, 305(1), 133-144.
Mouse Anti-ANTXR1 Recombinant Antibody (SB20) in WB
Supernatants were harvested from 293 cells cotransfected with myc-tagged secreted extracellular TEM8 and either C5A, C5B, or C5C and then immunoprecipitated with either nonspecific IgG (IP-IgG) or anti-TEM8 antibody. Supernatants or immunoprecipitates were immunoblotted with an anti-myc antibody. Note that the protein encoded by the C5C construct containing the partial C5 domain is undetectable in TEM8 immunoprecipitates. ...View More
Nanda, A., Carson-Walter, E. B., Seaman, S., Barber, T. D., Stampfl, J., Singh, S., ... & St. Croix, B. (2004). TEM8 interacts with the cleaved C5 domain of collagen α3 (VI). Cancer research, 64(3), 817-820.

Summary

Host Animal
Mouse
Specificity
Human
Clone
SB20
Antibody Isotype
IgG1
Application
ICC, IF, IHC-P, WB

Basic Information

Immunogen
Immunizing mice with a synthetic peptide containing amino acids 481-497 of human TEM8.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1-3 μg/ml
IHC-P2-5 μg/ml
IF(ICC)1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 481-497

Target

Full Name
Anthrax Toxin Receptor 1
Introduction
ANTXR1 is a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agen
Entrez Gene ID
84168
UniProt ID
Q9H6X2
Alternative Names
Anthrax Toxin Receptor 1; Tumor Endothelial Marker 8; TEM8; ATR; Tumor Endothelial Marker 8 Precursor; Anthrax Toxin Receptor; 2310008J16Rik; 2810405N18Rik; GAPO;
Function
Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
Biological Process
Actin cytoskeleton reorganization Source: UniProtKB
Blood vessel development Source: Ensembl
Negative regulation of extracellular matrix assembly Source: Ensembl
Positive regulation of metallopeptidase activity Source: Ensembl
Reproductive process Source: Ensembl
Substrate adhesion-dependent cell spreading Source: UniProtKB
Toxin transport Source: GO_Central
Cellular Location
Cell membrane; Lamellipodium membrane; Filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
Involvement in disease
Hemangioma, capillary infantile (HCI): A condition characterized by dull red, firm, dome-shaped hemangiomas, sharply demarcated from surrounding skin, usually presenting at birth or occurring within the first two or three months of life. They result from highly proliferative, localized growth of capillary endothelium and generally undergo regression and involution without scarring.
GAPO syndrome (GAPOS): An autosomal recessive disease characterized by growth retardation, alopecia, failure of tooth eruption, and progressive optic atrophy in some patients.
Topology
Extracellular: 33-321 aa
Helical: 322-342 aa
Cytoplasmic: 343-564 aa
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For research use only. Not intended for any clinical use.

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