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Rabbit Anti-Afdn Recombinant Antibody (V2-61030) (CBMAB-L2703-YJ)


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Published Data
Rabbit Anti-Afdn Recombinant Antibody (CBYJL-2390) (CBMAB-L2703-YJ) in IHC
Coronal sections from the brains of E11 embryos were stained with anti-N-afadin (green), -Par3 (magenta), or -Cdc42 (red) antibodies and Hoechst 33342 (blue). Afadin, Par3, and Cdc42 are detectable at the apical surface in the heterozygous cortex at E11 (arrows). The loss of Rap1 GTPases leads to a reduction in the apical localization of these proteins in the cortex (arrowheads). ...View More
Shah, B., Lutter, D., Tsytsyura, Y., Glyvuk, N., Sakakibara, A., Klingauf, J., & Püschel, A. W. (2017). Rap1 GTPases are master regulators of neural cell polarity in the developing neocortex. Cerebral cortex, 27(2), 1253-1269.

Summary

Host Animal
Rabbit
Specificity
Mouse
Clone
V2-61030
Antibody Isotype
IgG
Application
WB, IHC-P

Basic Information

Immunogen
Synthetic peptide corresponding to Mouse Afadin aa 1800 to the C-terminus (C terminal).
Host Species
Rabbit
Specificity
Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1-2 μg/ml
IHC-P1-2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Afadin, Adherens Junction Formation Factor
Introduction
Afdn belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. Afdn may play a key role in the organization of epithelial structures of the embryonic ectoderm.
Entrez Gene ID
17356
UniProt ID
Q9QZQ1
Alternative Names
AF6; Af-6; Gm314; Mllt4; Afadin; I-afadin; S-afadin; 5033403D15Rik; afadin; myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; protein Af-6
Function
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions.
Biological Process
Adherens junction organization
Bicellular tight junction assembly
Cell adhesion
Cell-cell adhesion mediated by cadherin
Cell-cell signaling
Establishment of endothelial intestinal barrier
Establishment of protein localization to plasma membrane
Negative regulation of cell migration
Pore complex assembly
Positive regulation of cell-cell adhesion
Positive regulation of cell-cell adhesion mediated by cadherin
Positive regulation of gene expression
Positive regulation of GTPase activity
Regulation of protein localization
Signal transduction
Cellular Location
Adherens junction. Not found at cell-matrix AJs.
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For research use only. Not intended for any clinical use.

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