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Mouse Anti-AFDN Recombinant Antibody (V2-58751) (CBMAB-L0408-YJ)


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Published Data
Mouse Anti-AFDN Recombinant Antibody (CBYJL-1002) (CBMAB-L0408-YJ) in IF
The F-actin disorganization associated with downregulation of nectin-3 in pan-RAR antagonist-treated testes. (A, D): Histological sections of testes from 8-week-old adult wild-type control mice treated for 7-days with vehicle alone. (B and C, E and F): Histological sections of testes from 8-week-old mice treated with pan-RAR antagonist at 5 mg/kg for 3 days or 7 days and euthanized one-day after dosing. (A−F): Original magnification, ×60. Left panel: localization of nectin-3 (red); middle panel: localization of F-actin (green); right panel: co-localization of nectin-3 (red), F-actin (green) and DNA (blue). The Arabic numerals indicate the step of spermatid differentiation. The red arrows in (B, C, E) point to the spermatids without nectin staining while the yellow arrows in C point to the retained spermatids without nectin-3 staining. The red brackets in (F) point to the step-9 spermatids without nectin-3 staining. A graph showing the relative fluorescence intensity of nectin-3 staining in drug-treated vs. the control vehicle group, illustrating an ~ 80% downregulation. Scale bar, 50 μm. ...View More
Chung, S. S., Vizcarra, N., & Wolgemuth, D. J. (2020). Filamentous actin disorganization and absence of apical ectoplasmic specialization disassembly during spermiation upon interference with retinoid signaling. Biology of Reproduction, 103(2), 378-389.
Mouse Anti-AFDN Recombinant Antibody (CBYJL-1002) (CBMAB-L0408-YJ) in FC
DC8 IT4var19, but not DC13 HB3var03 and IT4var07, IE binding to the HBEC line HBEC-5i is reduced by EPCR siRNA knockdown. (A) Expression of EPCR on HBEC-5i by immunostaining and flow cytometry. HBEC-5i was transfected with EPCR siRNA (orange) or a negative control siRNA (blue). Forty-eight hours after transfection, HBEC-5i was stained with 1 μg/mL goat polyclonal antibody to EPCR (orange/blue) or goat IgG-negative control (red), followed by Alexa Fluor 488 donkey anti-goat IgG at a 1:1,500 dilution. Data shown are representative of four similar experiments. (B-D) Binding of IEs to HBEC-5i transfected with control siRNA or EPCR siRNA under static conditions. The data shown are the mean and SEM from four independent experiments. The difference in mean values compared with the siRNA control was analyzed by a two-tailed paired t test. *P < 0.05. NS, not significant. ...View More
Azasi, Y., Lindergard, G., Ghumra, A., Mu, J., Miller, L. H., & Rowe, J. A. (2018). Infected erythrocytes expressing DC13 PfEMP1 differ from recombinant proteins in EPCR-binding function. Proceedings of the National Academy of Sciences, 115(5), 1063-1068.

Summary

Host Animal
Mouse
Specificity
Human, Rat, Dog, Mouse
Clone
V2-58751
Antibody Isotype
IgG1
Application
IHC-F, IA, IP, WB

Basic Information

Host Species
Mouse
Specificity
Human, Rat, Dog, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% BSA
Preservative
0.02% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Afadin, Adherens Junction Formation Factor
Introduction
AFDN involved in signaling and organization of cell junctions during embryogenesis. AFDN has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene, however, not all have been fully characterized.
Entrez Gene ID
Human4301
Mouse17356
Rat26955
Dog431695
UniProt ID
HumanP55196
MouseQ9QZQ1
RatO35889
DogF1PSU6
Alternative Names
AF6; MLLT4; MLL-AF6; l-afadin
Function
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions.
Biological Process
Adherens junction organization
Bicellular tight junction assembly
Cell adhesion
Cell-cell adhesion mediated by cadherin
Cell-cell signaling
Establishment of endothelial intestinal barrier
Establishment of protein localization to plasma membrane
Negative regulation of cell migration
Pore complex assembly
Positive regulation of cell-cell adhesion
Positive regulation of cell-cell adhesion mediated by cadherin
Positive regulation of gene expression
Positive regulation of GTPase activity
Regulation of protein localization
Signal transduction
Cellular Location
Adherens junction. Not found at cell-matrix AJs.
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For research use only. Not intended for any clinical use.

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