Rat Anti-ADGRE2 Recombinant Antibody (V2-607126) (CBMAB-C9458-LY)

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Datasheet

Summary

Host Animal

Rat

Specificity

Human

Clone

V2-607126

Antibody Isotype

IgG2a, κ

Application

IHC-F, FC

Basic Information

Immunogen

Recombinant protein fragment containing the first extracellular region of human EMR2 (24-540 a.a.)

Host Species

Rat

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-F	5-10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.2

Preservative

0.09% sodium azide

Concentration

0.5 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Adhesion G Protein-Coupled Receptor E2

Introduction

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Entrez Gene ID

30817

UniProt ID

Q9UHX3

Alternative Names

Adhesion G Protein-Coupled Receptor E2; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 2; EGF-Like Module-Containing Mucin-Like Hormone Receptor-Like 2; EMR2; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 2; EGF-Like Module Receptor 2; CD312 Antigen; CD312; VBU;

Function

Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cell adhesion
Cell migration
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Granulocyte chemotaxis
Inflammatory response
Regulation of mast cell degranulation

Cellular Location

Cell membrane; Ruffle membrane. Localized at the leading edge of migrating cells.

Involvement in disease

Vibratory urticaria (VBU): An autosomal dominant disorder characterized by localized hives and systemic manifestations in response to dermal vibration, with coincident degranulation of mast cells and increased histamine levels in serum.

Topology

Extracellular: 24-540 aa
Helical: 541-561 aa
Cytoplasmic: 562-569 aa
Helical: 570-590 aa
Extracellular: 591-605 aa
Helical: 606-626 aa
Cytoplasmic: 627-644 aa
Helical: 645-665 aa
Extracellular: 666-683 aa
Helical: 684-704 aa
Cytoplasmic: 705-735 aa
Helical: 736-756 aa
Extracellular: 757-760 aa
Helical: 761-781 aa
Cytoplasmic: 782-823 aa

PTM

Autoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane beta subunit.

More Infomation

References

Tseng, W. Y., Wang, W. C., Gordon, S., Ng, K. F., & Lin, H. H. (2021). Stimulation of Vibratory Urticaria-Associated Adhesion-GPCR, EMR2/ADGRE2, Triggers the NLRP3 Inflammasome Activation Signal in Human Monocytes. Frontiers in Immunology, 11, 3359.

Bhudia, N., Desai, S., King, N., Ancellin, N., Grillot, D., Barnes, A. A., & Dowell, S. J. (2020). G protein-coupling of adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and activation of G protein signalling by an anti-EMR2 antibody. Scientific reports, 10(1), 1-13.

Bhudia, N., Desai, S., King, N., Ancellin, N., Grillot, D., Barnes, A. A., & Dowell, S. J. (2020). Author correction: G protein-coupling of adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and activation of G protein signalling by an anti-EMR2 antibody. Scientific reports, 10(1), 1-2.

Naranjo, A. N., Bandara, G., Bai, Y., Smelkinson, M. G., Tobío, A., Komarow, H. D., ... & Olivera, A. (2020). Critical Signaling Events in the Mechanoactivation of Human Mast Cells through p. C492Y-ADGRE2. Journal of Investigative Dermatology, 140(11), 2210-2220.

Huang, Y. S., Hu, C. H., Tseng, W. Y., Cheng, C. H., Stacey, M., Gordon, S., ... & Lin, H. H. (2017). Activation of Adhesion GPCR EMR2/ADGRE2 Induces Macrophage Differentiation and Inflammatory Responses via Gα16/Akt/MAPK/NF-κB Signaling Pathways. Frontiers in immunology, 8, 373.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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