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Mouse Anti-ACTN4 Recombinant Antibody (V2-12513) (CBMAB-1003-CN)


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Published Data
Mouse Anti-ACTN4 Recombinant Antibody (7H6) (CBMAB-1003-CN) in WB
Cell-surface localization of gp96 and BiP in HEK293F cells. Following cell-surface biotinylation (with (+) or without (−) prior trypsinization), Western blotting of streptavidin-agarose-purified proteins with the indicated antibodies demonstrates gp96, BiP, and ADAMTS9 Pro-Cat (A), but not α-actinin-4 (B) at the cell-surface. C, gp96 and BiP form complexes with Pro-Cat or furin at the cell surface. Cell-surface proteins were cross-linked with DTSSP or BS3 on ice. As a control, cell-surface proteins were eliminated by prior (DTSSP) or subsequent (BS3) treatment with trypsin. Cell lysates were immunoprecipitated with polyclonal anti-Myc, and eluted proteins were analyzed by Western blotting with anti-gp96, anti-BiP, or monoclonal anti-Myc. Note that similar high molecular weight forms (>250 kDa) with BS3 are recognized by all three antibodies, and the expected gp96, BiP, and Pro-Cat molecular species (arrowheads) disappear or are decreased when cross-linked with BS3. ...View More
Koo, B. H., & Apte, S. S. (2010). Cell-surface processing of the metalloprotease pro-ADAMTS9 is influenced by the chaperone GRP94/gp96. Journal of Biological Chemistry, 285(1), 197-205.

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
V2-12513
Antibody Isotype
IgG
Application
WB, ELISA, IHC-F, IHC, IHC-P, IF

Basic Information

Immunogen
Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB2 μg/ml
IHC-P4 μg/ml
IF(ICC)4 μg/ml
ELISA1:100-1:2,000
FC1 μg / 10^6 cells
IHC1:20

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 1 mg/ml BSA
Preservative
0.05mg sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Actinin Alpha 4
Introduction
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). The protein is probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). It is involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). The protein may also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA
Entrez Gene ID
Human81
Mouse60595
UniProt ID
HumanO43707
MouseP57780
Alternative Names
FSGS; FSGS1; ACTININ-4
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
Biological Process
Negative regulation of substrate adhesion-dependent cell spreading
Peroxisome proliferator activated receptor signaling pathway
Platelet degranulation
Positive regulation of cell migration
Positive regulation of cellular component movement
Positive regulation of NIK/NF-kappaB signaling
Positive regulation of sodium:proton antiporter activity
Protein transport
Regulation of apoptotic process
Regulation of nucleic acid-templated transcription
Response to hypoxia
Retinoic acid receptor signaling pathway
Tumor necrosis factor-mediated signaling pathway
Vesicle transport along actin filament
Cellular Location
Nucleus; Cytoplasm; Stress fiber; Cell junction. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7).
Involvement in disease
Focal segmental glomerulosclerosis 1 (FSGS1): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
More Infomation

Shao, H., & Wells, A. (2021). Binding of alpha-ACTN4 to EGF receptor enables its rapid phosphorylation. Heliyon, 7(1), e06011.

Xing, Z., Li, S., Liu, Z., Zhang, C., & Bai, Z. (2020). CTCF‐induced upregulation of HOXA11‐AS facilitates cell proliferation and migration by targeting miR‐518b/ACTN4 axis in prostate cancer. The Prostate, 80(5), 388-398.

Ishizuya, Y., Uemura, M., Narumi, R., Tomiyama, E., Koh, Y., Matsushita, M., ... & Nonomura, N. (2020). The role of actinin-4 (ACTN4) in exosomes as a potential novel therapeutic target in castration-resistant prostate cancer. Biochemical and biophysical research communications, 523(3), 588-594.

Lin, Y. H., Huang, C. Y., Ke, C. C., Wang, Y. Y., Lai, T. H., Liu, H. C., ... & Lin, Y. H. (2020). ACTN4 mediates SEPT14 mutation-induced sperm head defects. Biomedicines, 8(11), 518.

Tentler, D., Lomert, E., Novitskaya, K., & Barlev, N. A. (2019). Role of ACTN4 in Tumorigenesis, Metastasis, and EMT. Cells, 8(11), 1427.

Ji, J., Xu, R., Ding, K., Bao, G., Zhang, X., Huang, B., ... & Wang, J. (2019). Long noncoding RNA SChLAP1 forms a growth-promoting complex with HNRNPL in human glioblastoma through stabilization of ACTN4 and activation of NF-κB signaling. Clinical Cancer Research, 25(22), 6868-6881.

Zhao, J., Peng, W., Ran, Y., Ge, H., Zhang, C., Zou, H., ... & Qi, H. (2019). Dysregulated expression of ACTN4 contributes to endothelial cell injury via the activation of the p38-MAPK/p53 apoptosis pathway in preeclampsia. Journal of physiology and biochemistry, 75(4), 475-487.

Zhang, Y. Y., Tabataba, H., Liu, X. Y., Wang, J. Y., Yan, X. G., Farrelly, M., ... & Jin, L. (2018). ACTN4 regulates the stability of RIPK1 in melanoma. Oncogene, 37(29), 4033-4045.

Lomert, E., Turoverova, L., Kriger, D., Aksenov, N. D., Nikotina, A. D., Petukhov, A., ... & Tentler, D. (2018). Co-expression of RelA/p65 and ACTN4 induces apoptosis in non-small lung carcinoma cells. Cell Cycle, 17(5), 616-626.

Kakuya, T., Mori, T., Yoshimoto, S., Watabe, Y., Miura, N., Shoji, H., ... & Honda, K. (2017). Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer. International journal of oral and maxillofacial surgery, 46(8), 968-976.

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For research use only. Not intended for any clinical use.

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