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Rabbit Anti-ACTN4 Recombinant Antibody (V2-179650) (CBMAB-A1001-YC)


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Published Data
Rabbit Anti-ACTN4 Recombinant Antibody (CBYC-A262) (CBMAB-A1001-YC) in IF
LED-Red irradiation promotes cardiomyocyte proliferation. (A) EdU and (B) pH3 staining were used to detect the proliferative effect of LED-Red on CMs. The data represent the mean ± SEM. ***P < 0.001 vs. Non-LED. Scale bar = 20 µm. ...View More
Gao, X., Li, H., Wang, X., Ren, Z., Tian, Y., Zhao, J., ... & Liu, Y. (2022). Light emitting diodes irradiation regulates miRNA-877-3p to promote cardiomyocyte proliferation. International Journal of Medical Sciences, 19(8), 1254.
Rabbit Anti-ACTN4 Recombinant Antibody (CBYC-A262) (CBMAB-A1001-YC) in IHC
Lack of ANGPTL3 inhibited the decreased expression of ACTN4 in podocytes after high-fat feeding. a The staining intensity of ACTN4 is shown in the four groups; b the staining intensity gradually decreased in the WT + HF group compared to the WT group. In contrast, the staining intensity did not decline in KO + HF mice. (Bars = 20 μm) ...View More
Li, G., Lu, D., Wang, J., Yue, S., Tan, M., Liu, M., & Gao, X. (2022). ANGPTL3 is involved in kidney injury in high-fat diet-fed mice by suppressing ACTN4 expression. Lipids in Health and Disease, 21(1), 90.
Rabbit Anti-ACTN4 Recombinant Antibody (CBYC-A262) (CBMAB-A1001-YC) in WB
Marker proteins of lEVs and sEVs analyzed by western blot analysis. ...View More
Wang, X., Meng, K., Wang, H., Wang, Y., Zhao, Y., Kang, J., ... & Quan, F. (2021). Identification of small extracellular vesicle subtypes in follicular fluid: Insights into the function and miRNA profiles. Journal of Cellular Physiology, 236(8), 5633-5645.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-179650
Antibody Isotype
IgG
Application
WB, IP, IHC-P, ICC

Basic Information

Immunogen
Synthetic peptide.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:10,000
IHC-P1:100-1:250
IF(ICC)1:100-1:250
FC1:50
IP1:30

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.2, 0.05% BSA, 40% Glycerol
Preservative
0.01% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Actinin Alpha 4
Introduction
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types
Entrez Gene ID
Human81
Mouse60595
Rat63836
UniProt ID
HumanO43707
MouseP57780
RatQ9QXQ0
Alternative Names
Actinin Alpha 4; Focal Segmental Glomerulosclerosis 1; Non-Muscle Alpha-Actinin 4; Alpha-Actinin-4; ACTININ-4; FSGS1; FSGS;
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
Biological Process
Negative regulation of substrate adhesion-dependent cell spreading
Peroxisome proliferator activated receptor signaling pathway
Platelet degranulation
Positive regulation of cell migration
Positive regulation of cellular component movement
Positive regulation of NIK/NF-kappaB signaling
Positive regulation of sodium:proton antiporter activity
Protein transport
Regulation of apoptotic process
Regulation of nucleic acid-templated transcription
Response to hypoxia
Retinoic acid receptor signaling pathway
Tumor necrosis factor-mediated signaling pathway
Vesicle transport along actin filament
Cellular Location
Nucleus; Cytoplasm; Stress fiber; Cell junction. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7).
Involvement in disease
Focal segmental glomerulosclerosis 1 (FSGS1): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
More Infomation

Shao, H., & Wells, A. (2021). Binding of alpha-ACTN4 to EGF receptor enables its rapid phosphorylation. Heliyon, 7(1), e06011.

Xing, Z., Li, S., Liu, Z., Zhang, C., & Bai, Z. (2020). CTCF‐induced upregulation of HOXA11‐AS facilitates cell proliferation and migration by targeting miR‐518b/ACTN4 axis in prostate cancer. The Prostate, 80(5), 388-398.

Ishizuya, Y., Uemura, M., Narumi, R., Tomiyama, E., Koh, Y., Matsushita, M., ... & Nonomura, N. (2020). The role of actinin-4 (ACTN4) in exosomes as a potential novel therapeutic target in castration-resistant prostate cancer. Biochemical and biophysical research communications, 523(3), 588-594.

Lin, Y. H., Huang, C. Y., Ke, C. C., Wang, Y. Y., Lai, T. H., Liu, H. C., ... & Lin, Y. H. (2020). ACTN4 mediates SEPT14 mutation-induced sperm head defects. Biomedicines, 8(11), 518.

Tentler, D., Lomert, E., Novitskaya, K., & Barlev, N. A. (2019). Role of ACTN4 in Tumorigenesis, Metastasis, and EMT. Cells, 8(11), 1427.

Ji, J., Xu, R., Ding, K., Bao, G., Zhang, X., Huang, B., ... & Wang, J. (2019). Long noncoding RNA SChLAP1 forms a growth-promoting complex with HNRNPL in human glioblastoma through stabilization of ACTN4 and activation of NF-κB signaling. Clinical Cancer Research, 25(22), 6868-6881.

Zhao, J., Peng, W., Ran, Y., Ge, H., Zhang, C., Zou, H., ... & Qi, H. (2019). Dysregulated expression of ACTN4 contributes to endothelial cell injury via the activation of the p38-MAPK/p53 apoptosis pathway in preeclampsia. Journal of physiology and biochemistry, 75(4), 475-487.

Zhang, Y. Y., Tabataba, H., Liu, X. Y., Wang, J. Y., Yan, X. G., Farrelly, M., ... & Jin, L. (2018). ACTN4 regulates the stability of RIPK1 in melanoma. Oncogene, 37(29), 4033-4045.

Lomert, E., Turoverova, L., Kriger, D., Aksenov, N. D., Nikotina, A. D., Petukhov, A., ... & Tentler, D. (2018). Co-expression of RelA/p65 and ACTN4 induces apoptosis in non-small lung carcinoma cells. Cell Cycle, 17(5), 616-626.

Kakuya, T., Mori, T., Yoshimoto, S., Watabe, Y., Miura, N., Shoji, H., ... & Honda, K. (2017). Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer. International journal of oral and maxillofacial surgery, 46(8), 968-976.

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For research use only. Not intended for any clinical use.

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