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Human Th1/Th2 Antibody Array (Glass Slide, 10 Targets) (Y0104) (AbAr-0104-YC)


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Specifications

Target Array
Th1/Th2 Array
Target
GM-CSF, IL-4, IFN-gamma, IL-5, IL-10, IL-6, IL-13, IL-8 (CXCL8), IL-2, TNF alpha
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
CD4 T-cells can be subdivided into Th1, Th2, Th17, and Treg subsets on the basis of their pattern of cytokine production. Protective Th1-related cytokines (Interleukin (IL)-2 and interferon (IFN)-γ) are involved in cellular immune responses. Th2-related cytokines (IL-4 and IL-10) are associated with humoral immunity and anti-inflammatory properties. However, these arise in a later period of periodontitis and are involved in the chronic progression of the disease. Th17 was identified as cells that recruited neutrophils and macrophages to participate and amplify the inflammatory reaction.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Second antibody and isotype control

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