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Human T Cell Response Antibody Array (Membrane, 20 Targets) (Y0574) (AbAr-0574-YC)


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Specifications

Target Array
T Cell Response Array
Target
IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-13, IFN-gamma, TGF beta 1, TNF alpha, IL-17A, IL-9, IL-22, IL-17F, IL-18, IL-23 p19, IL-21, IL-27, IL-33(IL-1 F11)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Cell-mediated immune responses involve the destruction of infected cells by cytotoxic T cells, or the destruction of intracellular pathogens by macrophages. The activation of naive T cells in response to antigen, and their subsequent proliferation and differentiation, constitutes a primary immune response.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Second antibody and isotype control

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