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NFKB2

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
Full Name
NFKB2
Function
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
Biological Process
Extracellular matrix organizationIEA:Ensembl
Follicular dendritic cell differentiationIEA:Ensembl
Germinal center formationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIIC:ComplexPortal
NIK/NF-kappaB signalingIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Rhythmic processIEA:UniProtKB-KW
Spleen developmentIEA:Ensembl
Cellular Location
Nucleus
Cytoplasm
Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Involvement in disease
Immunodeficiency, common variable, 10 (CVID10):
A primary immunodeficiency characterized by childhood-onset of recurrent infections, hypogammaglobulinemia, and decreased numbers of memory and marginal zone B-cells. Some patients may develop autoimmune features and have circulating autoantibodies. An unusual feature is central adrenal insufficiency.
PTM
While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing.
Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.

Anti-NFKB2 antibodies

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Target: NFKB2
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: 9BA14
Application*: E
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 7H5
Application*: IP, WB, M
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 1E1
Application*: IP, WB, M
Target: NFKB2
Host: Rat
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: CBWJN-0807
Application*: WB, IF
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBWJN-0776
Application*: IP, WB
Target: NFKB2
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse, Rat, Human
Clone: CBWJN-1377
Application*: WB, IP, F, IC
Target: NFKB2
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: E.212.1
Application*: F, P, WB
Target: NFKB2
Host: Mouse
Specificity: Human, Mouse, Rat
Clone: C-5
Application*: WB, IP, IF, F
Target: NFKB2
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBWJN-0533
Application*: WB, IC, F
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Rat
Clone: 9D2
Application*: E, F, WB
Target: NFkB2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBWJN-0492
Application*: DB, WB
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 7H260
Application*: IC, WB, CI
Target: NFKB2
Host: Rabbit
Antibody Isotype: IgG
Specificity: Hamster, Human, Monkey, Mouse, Pig, Rat
Clone: CBWJN-0420
Application*: WB, IP, F, IF
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG3
Specificity: Human
Clone: 2Q2254
Application*: E, WB, IH, IC, F, IF
Target: NFKB2
Host: Rabbit
Specificity: Human, Monkey
Clone: 18D10
Application*: WB, P, F
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBWJN-1376
Application*: IC, IP, WB
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: 4H211
Application*: WB, F
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBWJN-0396
Application*: DB, WB
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBWJN-0986
Application*: WB, IP
Target: NFKB2
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: D7A9K
Application*: WB, IP, CI
Target: NFKB2
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse, Rat
Clone: D9S3M
Application*: WB, IP
Target: NFKB2
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: CBYCL-617
Application*: WB, IC, P, C, E
More Infomation
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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