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CDK2

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
Full Name
Cyclin Dependent Kinase 2
Function
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity).
Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity).
Phosphorylates CDK2AP2 (PubMed:12944431).
Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878).
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: Reactome
Cell division Source: UniProtKB-KW
Cellular response to nitric oxide Source: UniProtKB
Centriole replication Source: UniProtKB
Centrosome duplication Source: UniProtKB
DNA repair Source: UniProtKB-KW
DNA replication Source: UniProtKB
DNA replication initiation Source: Reactome
G1/S transition of mitotic cell cycle Source: GO_Central
G2/M transition of mitotic cell cycle Source: Reactome
Granulocyte differentiation Source: Reactome
Histone phosphorylation Source: CAFA
Meiotic cell cycle Source: UniProtKB
Mitotic G1 DNA damage checkpoint Source: UniProtKB
Peptidyl-serine phosphorylation Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Ras protein signal transduction Source: BHF-UCL
Regulation of G2/M transition of mitotic cell cycle Source: GO_Central
Regulation of gene expression Source: GO_Central
Regulation of gene silencing Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: Reactome
Signal transduction Source: GO_Central
Telomere maintenance via telomerase Source: Reactome
Cellular Location
Centrosome; Cajal body; Cytoplasm; Endosome. Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)2D3.
PTM
Phosphorylated at Thr-160 by CDK7 in a CAK complex (PubMed:28666995). Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.
Nitrosylated after treatment with nitric oxide (DETA-NO).

Anti-CDK2 antibodies

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Target: CDK2
Host: Rabbit
Antibody Isotype: IgG
Specificity: Fruit fly, Human, Mouse, Rat
Clone: CBNH-128
Application*: WB, E
Target: CDK2
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human, Mouse, Frog
Clone: CBYY-C1759
Application*: WB, IP, E, F
Target: CDK2
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Frog
Clone: CBYY-C1758
Application*: E, IF, IH, WB
Target: CDK2
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Rat
Clone: CBYY-C0324
Application*: WB, IF
Target: CDK2
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse, Rat
Clone: CBFYC-1595
Application*: WB, IP
Target: CDK2
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse, Rat
Clone: CBFYC-1594
Application*: E, IF, IP, WB
Target: CDK2
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse, Rat
Clone: CBFYC-1593
Application*: WB, IP
Target: CDK2
Host: Mouse
Antibody Isotype: IgG1, λ
Specificity: Human
Clone: 3A2-3G6
Application*: E, IP, WB
Target: CDK2
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 2E8
Application*: WB, E
More Infomation
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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