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Mouse RTK Phosphorylation Antibody Array (Membrane, 39 Targets) (Y0237) (AbAr-0237-YC)


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Specifications

Target Array
RTK Phosphorylation Array
Target
EGF R, PDGF R alpha, VEGF R3, ErbB2, PDGF R beta, MuSK, ErbB3, SCF R, EphA1, ErbB4, Flt-3, EphA2, FGF R2 (IIIc), M-CSF R, EphA3, FGF R3, c-Ret, EphA6, FGF R4, Tie-1, EphA7, Insulin R, Tie-2, EphA8, IGF-I R, TrkA, EphB1, Axl, TrkB, EphB2, DtK, TrkC, EphB4, Mer, VEGF R1, EphB6, HGF R, VEGF R2, MSP R
Species Reactivity
Mouse
Sample Type
Cell Lysates

Applications

Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. Of the 90 unique tyrosine kinase genes identified in the human genome, 58 encode receptor tyrosine kinase proteins. Receptor tyrosine kinases have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Mutations in receptor tyrosine kinases lead to activation of a series of signalling cascades which have numerous effects on protein expression. Receptor tyrosine kinases are part of the larger family of protein tyrosine kinases, encompassing the receptor tyrosine kinase proteins which contain a transmembrane domain, as well as the non receptor tyrosine kinases which do not possess transmembrane domains.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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