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Human Phosphorylation Pathway Antibody Array (Membrane, 55 Targets) (Y0566) (AbAr-0566-YC)


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Specifications

Target Array
Phosphorylation Pathway Array
Target
4E-BP1 (P-Thr36), AKT (P-Ser473), AMPKa (P-Thr172), ATF2 (P-Thr69/71), ATM (P-Ser1981), BAD (P-Ser112), C-Fos (P-Thr232), c-Jun (P-Ser73), CREB (P-Ser133), EGFR (P-Ser1070), eIF-2a (P-Ser51), ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187), GSK-3 alpha (P-Ser21), GSK-3 beta (P-Ser9), HDAC2 (P-Ser394), HDAC4 (P-Ser632), HSP27 (P-Ser82), IKBa (P-Ser32), JAK1 (P-Tyr1022), JAK2 (P-Tyr1007/1008), JNK (P-Thr183), MEK (P-Ser217/221), MKK3 (P-Ser189), MKK6 (P-Ser207)(MEK6), MSK1 (P-Ser376), MSK2 (P-Ser360), mTOR (P-Ser2448), NF-kB (P-Ser536), p27 (P-Thr198), p38 (PThr180/Tyr182), P53 (P-Ser15), P70S6K (P-Thr421/Ser424), PDK1 (P-Ser241), PRAS40 (P-Thr246), PTEN (P-Ser380), Raf-1 (P-Ser301), RPS6 (P-Ser235/236), RSK1 (P-Ser380), RSK2 (P-Ser386), SHP-1 (P-Ser591), SHP2 (P-Tyr542), SMAD1 (P-Ser463/465), SMAD2 (P-Ser245/250/255), SMAD4 (P-Thr277), SMAD5 (P-Ser463/465), Src (P-Tyr419), STAT1 (P-Ser727), STAT2 (P-Tyr689), STAT3 (P-Tyr705), STAT5 (P-Tyr694), STAT6 (P-Tyr641), TAK1 (P-Ser412), TBK1 (P-Ser172), TYK2 (P-Tyr1054), ZAP70 (P-Tyr292)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP Conjugated Anti-Rabbit IgG Antibodies
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Protein phosphorylation is considered the most abundant post-translational modification in eukaryotes. Phosphorylation can occur on serine, threonine and tyrosine side chains (often called 'residues') through phosphoester bond formation, on histidine, lysine and arginine through phosphoramidate bonds, and on aspartic acid and glutamic acid through mixed anhydride linkages. Recent evidence confirms widespread histidine phosphorylation at both the 1 and 3 N-atoms of the imidazole ring. Recent work demonstrates widespread human protein phosphorylation on multiple non-canonical amino acids, including motifs containing phosphorylated histidine, aspartate, glutamate, cysteine, arginine and lysine in HeLa cell extracts.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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