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Human Obesity Antibody Array (Glass Slide, 10 Targets) (Y0078) (AbAr-0078-YC)


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Specifications

Target Array
Obesity Array
Target
IGF-1, Leptin, IL-1 beta (IL-1 F2), MCP-1 (CCL2), IL-6, PAI-1, IL-8 (CXCL8), Resistin, Insulin, TNF alpha
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Obesity is linked not only with excessive accumulation of fat tissue, but with adverse effects on health. The placement of fatty tissue is also significant. Abdominal obesity is especially pathological. Excess of body weight increases the risk of cardiovascular diseases, diabetes mellitus type 2, obstructive sleep apnea syndrome, gallstones, renal diseases, some types of cancer (e.g. endometrial cancer), inflammation of bones and joints, therefore reducing the expected length of life. Severe obesity leads to disability. The most common cause of obesity is eating too big amounts of food. In addition, in the pathogenesis of overweight other factors are implemented – genetic, biological, pharmacological, environmental and psychological. Genetic factors play a role not only in promoting obesity, but increasing susceptibility to its development.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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