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Mouse Anti-1-methyladenosine (m1A) Recombinant Antibody (V2-9283) (CBMAB-1192-LY)


See all 1-methyladenosine (m1A) antibodies
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Published Data
Mouse Anti-1-methyladenosine (m1A) Recombinant Antibody (AMA-2) in Immunoblot
Immuno-northern blot analysis of mitochondrial and non-mitochondrial RNA.
RNAs were isolated from the total fraction (Total), mitochondrial fraction (Mito.), and non-mitochondrial fraction (Non-mito.) of mouse liver. (A) Each total RNA (0.6 μg) was separated in the 12% polyacrylamide gel and analyzed by SYBR staining and INB with anti-m1A antibody. The red arrowheads denote shorter tRNA bands, which were rich in the mitochondrial RNA. Arrows denote slight anti-m1A positive bands in the long exposure image. White arrowheads denote negative signals in 5.8S and 5S rRNA. (B) Each total RNA (1.2 μg) was separated on the 1% agarose gel and analyzed. The arrowheads denote positive signals in 28S and 18S rRNA. The arrow probably denotes the 28S rRNA-derived degraded transcript produced during the sample preparation. ...View More
Mishima, E., Jinno, D., Akiyama, Y., Itoh, K., Nankumo, S., Shima, H., ... & Abe, T. (2015). Immuno-northern blotting: detection of RNA modifications by using antibodies against modified nucleosides. PloS one, 10(11), e0143756.

Summary

Host Animal
Mouse
Clone
V2-9283
Antibody Isotype
IgG2b, κ
Application
IP, ICC, IHC, ELISA

Basic Information

Immunogen
KLH conjugated 1-methyladenosine
Host Species
Mouse
Antibody Isotype
IgG2b, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 50% Glycerol
Preservative
None
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
1-methyladenosine (m1A)
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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