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Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0117) (AbAr-0117-YC)


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Specifications

Target Array
Interleukin Array
Target
GCSF, IL-10, IL-2, IL-5, GM-CSF, IL-12 p70, IL-21, IL-6, IFN-gamma, IL-13, IL-23 p19, IL-7, IL-1 alpha (IL-1 F1), IL-15, IL-3, IL-9, IL-1 beta (IL-1 F2), IL-17A, IL-4, TNF alpha
Species Reactivity
Mouse
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Interleukins (ILs) are a group of cytokines (secreted proteins and signal molecules) that were first seen to be expressed by white blood cells (leukocytes). ILs can be divided into four major groups based on distinguishing structural features. However, their amino acid sequence similarity is rather weak (typically 15–25% identity). The human genome encodes more than 50 interleukins and related proteins. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4 T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T and B lymphocytes, and hematopoietic cells.
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Alternative Versions

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553)

Canine Interleukin Antibody Array (Membrane, 11 Targets) (Y0552)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116)

Antibody Array

Canine Interleukin Antibody Array (Membrane, 11 Targets) (Y0552)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557)

Related Products

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0118)

Mouse Interleukin Antibody Array (Glass Slide, 20 Targets) (Y0557)

Bovine Interleukin Antibody Array (Membrane, 11 Targets) (Y0553)

Mouse Interleukin Antibody Array (Glass Slide, 10 Targets) (Y0555)

Canine Interleukin Antibody Array (Membrane, 11 Targets) (Y0552)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0116)

Porcine Interleukin Antibody Array (Membrane, 16 Targets) (Y0554)

Mouse Interleukin Antibody Array (Glass Slide, 30 Targets) (Y0556)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Second antibody and isotype control

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