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Human IGF Signaling Antibody Array (Glass Slide, 10 Targets) (Y0098) (AbAr-0098-YC)


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Specifications

Target Array
IGF Signaling Array
Target
IGF-1, IGFBP-2, IGF-1 R, IGFBP-3, IGF-2, IGFBP-4, IGF-2 R, IGFBP-6, IGFBP-1, Insulin
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Thoroughly dry the glass slide and block the array surface to prevent non-specific binding.
Incubate the array with both samples and standards.
Incubate with a biotinylated detection antibody cocktail and then with streptavidin-conjugated fluorescent dye.
Carefully disassemble the glass slide and scan the slide using a gene microarray laser scanner.
Conduct densitometric analysis.
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
The insulin-like growth factor (IGF) signaling axis is critical to the growth, development, and maintenance of many tissues within the human body. It is particularly important during neonatal and pubertal growth, and essentially carries out its effects by simulating cell proliferation and interrupting programmed cell death. The IGF system is comprised of two ligands, IGF-1 and IGF-2, which exhibit their effects through binding to IGF-1R (primarily), IGF-2R, and the insulin receptor (IR), all belonging to the tyrosine kinase receptor family.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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