Rat Growth Factor Antibody Array (Membrane, 14 Targets) (Y0538) (AbAr-0538-YC)

See all Growth Factor antibodies

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Datasheet

Specifications

Target Array

Growth Factor Array

Target

Activin A, BDNF, beta-NGF, bFGF, EGF, Flt-3 Ligand, GDNF, GM-CSF, HGF, IGF-1, PDGF-AA, PDGF-BB, IGFBP-5, VEGF-A

Species Reactivity

Rat

Sample Type

Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application

Multiplexed Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Membrane

Detection Assay

Sandwich-based Assay

Protocol Outline

Block the array membrane surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data

Components

Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction

A growth factor is a naturally occurring substance capable of stimulating cellular growth, proliferation, healing, and cellular differentiation. Usually it is a protein or a steroid hormone. Growth factors are important for regulating a variety of cellular processes. Growth factors typically act as signaling molecules between cells. Examples are cytokines and hormones that bind to specific receptors on the surface of their target cells. They often promote cell differentiation and maturation, which varies between growth factors. For example, epidermal growth factor (EGF) enhances osteogenic differentiation, while fibroblast growth factors and vascular endothelial growth factors stimulate blood vessel differentiation (angiogenesis).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0039)

Mouse Growth Factor Antibody Array (Membrane, 10 Targets) (Y0189)

Mouse Growth Factor Antibody Array (Membrane, 20 Targets) (Y0190)

Mouse Growth Factor Antibody Array (Membrane, 30 Targets) (Y0194)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0256)

Human Growth Factor Antibody Array (Glass Slide, 41 Targets) (Y0052)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0097)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0539)

Antibody Array

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0039)

Mouse Growth Factor Antibody Array (Membrane, 10 Targets) (Y0189)

Mouse Growth Factor Antibody Array (Membrane, 20 Targets) (Y0190)

Mouse Growth Factor Antibody Array (Membrane, 30 Targets) (Y0194)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0256)

Human Growth Factor Antibody Array (Glass Slide, 41 Targets) (Y0052)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0097)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0539)

Related Products

Human Growth Factor Antibody Array (Glass Slide, 41 Targets) (Y0052)

Mouse Growth Factor Antibody Array (Membrane, 10 Targets) (Y0189)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0256)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0039)

Mouse Growth Factor Antibody Array (Membrane, 30 Targets) (Y0194)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0097)

Mouse Growth Factor Antibody Array (Membrane, 20 Targets) (Y0190)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0539)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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Second antibody and isotype control

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