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Human Dry Eye Disease Antibody Array (Glass Slide, 40 Targets) (Y0320) (AbAr-0320-YC)


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Specifications

Target Array
Dry Eye Disease Array
Target
EGF, EGF R, Eotaxin, G-CSF, GRO, HB-EGF, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IP-10, Lipocalin-2, MCP-1, MIG, MIP-1α, MIP-1β, MIP-1δ, MMP-9, RANTES, TGFβ1, TIMP-1, TIMP-2, TNFα, VEGF
Species Reactivity
Human
Sample Type
Serum, Plasma, Other Biological Fluids, Cell Lysates, Cell Culture Media, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Label-based
Protocol Outline
Thoroughly dry the glass slide.
Incubate with the samples.
Incubate using a biotin lectin mixture and then with fluorescent dye-conjugated streptavidin.
Carefully disassemble the glass slide.
Utilize a gene microarray laser scanner for scanning.
Conduct densitometric analysis.
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Dry eye syndrome (DES), also known as keratoconjunctivitis sicca (KCS), is the condition of having dry eyes. Other associated symptoms include irritation, redness, discharge, and easily fatigued eyes. Dry eye occurs when either the eye does not produce enough tears or when the tears evaporate too quickly.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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