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Mouse Cytokine Antibody Array (Glass Slide, 48 Targets) (Y0073) (AbAr-0073-YC)


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Specifications

Target Array
Cytokine Array
Target
4-1BB (TNFRSF9/CD137), Cardiotrophin-1 (CT-1), Chordin, EGF, Gas 1, HGF, IL-17F, IL-28A (IFN-lambda 2), Neprilysin, TREM-1, 6Ckine (CCL21), CD27 (TNFRSF7), CTLA-4 (CD152), Endoglin (CD105), Gas 6, IL-1 R4 (ST2), IL-1 RA (IL-1 F3), IL-6 R, Pentraxin-3 (TSG-14), TWEAK (TNFSF12), ACE, CD27 Ligand (TNFSF7), Decorin, Epigen, GITR Ligand (TNFSF18), IL-11, IL-2 R alpha, JAM-A (CD321/F11R), Prolactin, TWEAK R (TNFRSF12), ALK-1, CD36 (SR-B3), DKK-1, Epiregulin, Granzyme B, IL-17B, IL-20, MAdCAM-1, RAGE, Amphiregulin, CD40 Ligand (TNFSF5), E-Cadherin, Galectin-1, HAI-1, IL-17E (IL-25), IL-21, MFG-E8, TACI (TNFRSF13B)
Species Reactivity
Mouse
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Cytokines are a broad and loose category of small proteins (5–20 kDa) that are important in cell signaling. Cytokines are peptides, and cannot cross the lipid bilayer of cells to enter the cytoplasm. Cytokines have been shown to be involved in autocrine signaling, paracrine signaling and endocrine signaling as immunomodulating agents. Their definite distinction from hormones is still part of ongoing research. Cytokines include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced by more than one type of cell.
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Alternative Versions

Human Cytokine Antibody Array (Glass Slide, 60 Targets) (Y0049)

Human Cytokine Antibody Array (Glass Slide, 60 Targets) (Y0038)

Human Cytokine Antibody Array (Glass Slide, 80 Targets) (Y0037)

Human Cytokine Antibody Array (Glass Slide, 274 Targets) (Y0036)

Human Cytokine Antibody Array (Glass Slide, 174 Targets) (Y0034)

Human Cytokine Antibody Array (Glass Slide, 49 Targets) (Y0033)

Human Cytokine Antibody Array (Glass Slide, 120 Targets) (Y0032)

Mouse Cytokine Antibody Array (Glass Slide, 144 Targets) (Y0069)

Mouse Cytokine Antibody Array (Membrane, 32 Targets) (Y0062)

Mouse Cytokine Antibody Array (Membrane, 144 Targets) (Y0061)

Antibody Array

Human Cytokine Antibody Array (Membrane, 120 Targets) (Y0020)

Human Cytokine Antibody Array (Membrane, 49 Targets) (Y0021)

Canine Cytokine Antibody Array (Membrane, 40 Targets) (Y0022)

Human Cytokine Antibody Array (Membrane, 174 Targets) (Y0023)

Human Cytokine Antibody Array (Membrane, 42 Targets) (Y0024)

Human Cytokine Antibody Array (Membrane, 274 Targets) (Y0025)

Human Cytokine Antibody Array (Membrane, 36 Targets) (Y0026)

Human Cytokine Antibody Array (Membrane, 80 Targets) (Y0027)

Human Cytokine Antibody Array (Membrane, 60 Targets) (Y0028)

Human Cytokine Antibody Array (Membrane, 60 Targets) (Y0029)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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