Human Cancer Discovery Antibody Array (Membrane, 10 Targets) (Y0191) (AbAr-0191-YC)

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Datasheet

Specifications

Target Array

Cancer Discovery Array

Target

Alpha-fetoprotein, HGF, CA125, Osteopontin (SPP1), CA19-9, PSA-free, CEA, TIMP-1, GDF-15, VEGF-A

Species Reactivity

Human

Sample Type

Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application

Multiplex Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Membrane

Detection Assay

Sandwich-based

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Components

Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction

Cytokines that are released in response to infection, inflammation and immunity can function to inhibit cancer development and progression. Alternatively, cancer cells can respond to host-derived cytokines that promote growth, attenuate apoptosis and facilitate invasion and metastasis.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Human Cancer Discovery Antibody Array (Membrane, 30 Targets) (Y0193)

Human Cancer Discovery Antibody Array (Membrane, 20 Targets) (Y0192)

Human Cancer Discovery Antibody Array (Glass Slide, 72 Targets) (Y0409)

Human Cancer Discovery Antibody Array (Glass Slide, 12 Targets) (Y0408)

Human Cancer Discovery Antibody Array (Glass Slide, 40 Targets) (Y0407)

Human Cancer Discovery Antibody Array (Glass Slide, 20 Targets) (Y0406)

Human Cancer Discovery Antibody Array (Glass Slide, 72 Targets) (Y0405)

Human Cancer Discovery Antibody Array (Glass Slide, 12 Targets) (Y0404)

Human Cancer Discovery Antibody Array (Glass Slide, 40 Targets) (Y0403)

Human Cancer Discovery Antibody Array (Glass Slide, 20 Targets) (Y0402)

Antibody Array

Human Cancer Discovery Antibody Array (Membrane, 20 Targets) (Y0192)

Human Cancer Discovery Antibody Array (Membrane, 30 Targets) (Y0193)

Human Cancer Discovery Antibody Array (Glass Slide, 20 Targets) (Y0402)

Human Cancer Discovery Antibody Array (Glass Slide, 40 Targets) (Y0403)

Human Cancer Discovery Antibody Array (Glass Slide, 12 Targets) (Y0404)

Human Cancer Discovery Antibody Array (Glass Slide, 72 Targets) (Y0405)

Human Cancer Discovery Antibody Array (Glass Slide, 20 Targets) (Y0406)

Human Cancer Discovery Antibody Array (Glass Slide, 40 Targets) (Y0407)

Human Cancer Discovery Antibody Array (Glass Slide, 12 Targets) (Y0408)

Human Cancer Discovery Antibody Array (Glass Slide, 72 Targets) (Y0409)

Related Products

Human Cancer Discovery Antibody Array (Glass Slide, 12 Targets) (Y0408)

Human Cancer Discovery Antibody Array (Membrane, 30 Targets) (Y0193)

Human Cancer Discovery Antibody Array (Glass Slide, 20 Targets) (Y0402)

Human Cancer Discovery Antibody Array (Glass Slide, 40 Targets) (Y0407)

Human Cancer Discovery Antibody Array (Membrane, 20 Targets) (Y0192)

Human Cancer Discovery Antibody Array (Glass Slide, 20 Targets) (Y0406)

Human Cancer Discovery Antibody Array (Glass Slide, 72 Targets) (Y0405)

Human Cancer Discovery Antibody Array (Glass Slide, 12 Targets) (Y0404)

Human Cancer Discovery Antibody Array (Glass Slide, 40 Targets) (Y0403)

Human Cancer Discovery Antibody Array (Glass Slide, 72 Targets) (Y0409)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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