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Human Atherosclerosis Antibody Array (Membrane, 20 Targets) (Y0007) (AbAr-0007-YC)


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Specifications

Target Array
Atherosclerosis Array
Target
CCL11, IFNG, CCL2, TNF alpha, GCSF, IL1A, M-CSF, TNFSF1B, GDNF, IL1B, TGF beta 1, CCL20, GM-CSF, IL-1 R1, TGF beta 2, PDGF-BB, CD54, IL-1 R2, TGF beta 3, CCL5
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Apply a blocking agent to the membranes.
Proceed with sample incubation.
Incubate using the cocktail of biotinylated detection antibodies.
Incubate with streptavidin conjugated to horseradish peroxidase (HRP).
Conduct incubation with the detection buffers.
Utilize a chemiluminescent imaging system for image capture.
Execute densitometric evaluation and perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Atherosclerosis is a disease in which plaque builds up inside your arteries. Arteries are blood vessels that carry oxygen-rich blood to your heart and other parts of your body. Plaque is made up of fat, cholesterol, calcium, and other substances found in the blood. Over time, plaque hardens and narrows your arteries.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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