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Human Apoptosis Antibody Array (Membrane, 35 Targets) (Y0233) (AbAr-0233-YC)


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Specifications

Target Array
Apoptosis Array
Target
Bad, TRAIL R1/DR4, PON2, Bax, TRAIL R2/DR5, p21/CIP1/CDNK1A, Bcl-2, FADD, p27/Kip1, Bcl-x, Fas/TNFSF6, Phospho-p53 (S15), Pro-Caspase-3, HIF-1 alpha, Phospho-p53 (S46), Cleaved Caspase-3, HO-1/HMOX1/HSP32, Phospho-p53 (S392), Catalase, HO-2/HMOX2, Phospho-Rad17 (S635), cIAP-1, HSP27, Pro-Caspase-3, cIAP-2, HSP60, SMAC/Diablo, Claspin, HSP70, Survivin, Clusterin, HTRA2/Omi, TNF RI/TNFRSF1A, Cytochrome c, Livin, XIAP
Species Reactivity
Human
Sample Type
Cell Lysates

Applications

Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. The average adult human loses between 50 and 70 billion cells each day due to apoptosis. For an average human child between the ages of 8 to 14 year old approximately 20 to 30 billion cells die per day.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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