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Mouse Anti-XPO5 Recombinant Antibody (2C5-1B3) (CBMAB-X0164-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2C5-1B3
Antibody Isotype
IgG1, Κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Full length recombinant corresponding to aa1-281 from human XPO5 (AAH00129) with GST tag.
Specificity
Human
Antibody Isotype
IgG1, Κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
Localized in the amino acids 1-281

Target

Full Name
Exportin 5
Introduction
XPO5 is a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. XPO5 mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form.
Entrez Gene ID
57510
UniProt ID
Q9HAV4
Alternative Names
exp5
Function
Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.
Mediates the nuclear export of micro-RNA precursors, which form short hairpins (PubMed:14681208, PubMed:14631048, PubMed:15613540).
Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs (PubMed:19965479).
Binding is dependent on Ran-GTP (PubMed:19965479).
(Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.
Biological Process
Biological Process miRNA metabolic process Source:Reactome
Biological Process positive regulation of post-transcriptional gene silencing by RNA Source:BHF-UCL1 Publication
Biological Process pre-miRNA export from nucleus Source:BHF-UCL1 Publication
Biological Process protein export from nucleus Source:UniProtKB1 Publication
Biological Process RNA export from nucleus Source:GO_Central1 Publication
Cellular Location
Nucleus
Cytoplasm
Shuttles between the nucleus and the cytoplasm.
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For research use only. Not intended for any clinical use.

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