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Rabbit Anti-VAMP8 Recombinant Antibody (BA0047) (CBMAB-0941CQ)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0047
Antibody Isotype
IgG
Application
FC, IF, IHC-P, IP, WB

Basic Information

Immunogen
Human VAMP8 (N terminal)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Vesicle Associated Membrane Protein 8
Introduction
This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
Entrez Gene ID
Human8673
Mouse22320
Rat83730
UniProt ID
HumanQ9BV40
MouseO70404
RatQ9WUF4
Alternative Names
EDB; VAMP-8
Function
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604).
Also required for dense-granule secretion in platelets (PubMed:12130530).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity).
Involved in the homotypic fusion of early and late endosomes (By similarity).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (PubMed:31694946).
Biological Process
Biological Process autophagosome maturation Source:UniProtKB1 Publication
Biological Process autophagosome membrane docking Source:GO_Central1 Publication
Biological Process defense response to virus Source:UniProtKB-KW
Biological Process mucus secretion Source:UniProtKB1 Publication
Biological Process negative regulation of secretion by cell Source:UniProtKB1 Publication
Biological Process positive regulation of histamine secretion by mast cell Source:UniProtKB1 Publication
Biological Process protein transport Source:UniProtKB-KW
Biological Process regulation of protein localization to plasma membrane Source:UniProtKB1 Publication
Biological Process SNARE complex assembly Source:GO_Central1 Publication
Biological Process vesicle fusion Source:GO_Central1 Publication
Biological Process viral entry into host cell Source:MGI1 Publication
Cellular Location
Lysosome membrane
Early endosome membrane
Late endosome membrane
Cell membrane
Zymogen granule membrane
Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity).
Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (By similarity).
Topology
Cytoplasmic: 1-75
Helical: 76-96
Vesicular: 97-100
PTM
(Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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