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Rabbit Anti-ULK1 Recombinant Antibody (D9D7) (CBMAB-C4603-CN)


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Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
D9D7
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Specificity
Human, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Target

Full Name
Unc-51 Like Autophagy Activating Kinase 1
Introduction
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Entrez Gene ID
Human8408
Monkey709746
UniProt ID
HumanO75385
MonkeyF6QIW8
Alternative Names
Unc-51 Like Autophagy Activating Kinase 1; Autophagy-Related Protein 1 Homolog; EC 2.7.11.1; HATG1; ATG1; ATG1 Autophagy Related 1 Homolog (S. Cerevisiae); Serine/Threonine-Protein Kinase ULK1; Unc-51 (C. Elegans)-Like Kinase 1; Unc-51-Like Kinase 1 (C. Elegans);
Function
Serine/threonine-protein kinase involved in autophagy in response to starvation (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:23524951, PubMed:25040165, PubMed:31123703).
Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes (PubMed:18936157, PubMed:21460634, PubMed:21795849, PubMed:25040165).
Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR (PubMed:21795849).
Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity (PubMed:21460634).
May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences (PubMed:18936157).
Plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed:11146101).
May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Phosphorylates FLCN, promoting autophagy (PubMed:25126726).
Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed:20921139).
Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B (PubMed:28821708).
Biological Process
Autophagosome assemblyManual Assertion Based On ExperimentIDA:UniProtKB
AutophagyManual Assertion Based On ExperimentIDA:UniProtKB
Autophagy of mitochondrionManual Assertion Based On ExperimentIBA:GO_Central
Axon extensionManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to nutrient levelsISS:UniProtKB
Late nucleophagyManual Assertion Based On ExperimentIBA:GO_Central
MacroautophagyManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIDA:ComplexPortal
Negative regulation of collateral sproutingManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of protein-containing complex assemblyManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Neuron projection developmentManual Assertion Based On ExperimentIMP:UniProtKB
Neuron projection regenerationIEA:Ensembl
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-threonine phosphorylationManual Assertion Based On ExperimentIDA:HGNC
Piecemeal microautophagy of the nucleusManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of autophagosome assemblyIEA:Ensembl
Positive regulation of autophagyManual Assertion Based On ExperimentIDA:UniProtKB
Protein autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein localizationManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:ComplexPortal
Regulation of macroautophagyManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of protein lipidationManual Assertion Based On ExperimentIDA:ComplexPortal
Response to starvationISS:UniProtKB
ReticulophagyManual Assertion Based On ExperimentIBA:GO_Central
Signal transductionIEA:InterPro
Cellular Location
Cytoplasm, cytosol
Preautophagosomal structure
Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
PTM
Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity).
In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
Ubiquitinated via 'Lys-63'-linkage by a complex composed of AMBRA1 and TRAF6 following autophagy induction, promoting ULK1 stability and kinase activity.
Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.
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For research use only. Not intended for any clinical use.

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