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Mouse Anti-UBE2W Recombinant Antibody (7G4) (CBMAB-A9733-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
7G4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
UBE2W (NP_001001481, 17 a.a. ~ 112 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ubiquitin-conjugating enzyme E2W (putative)
Entrez Gene ID
55284
UniProt ID
Q96B02
Alternative Names
FLJ11011; hUBC-16
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:20061386, PubMed:21229326).
Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini (PubMed:23560854, PubMed:23696636, PubMed:25436519).
Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:19111657, PubMed:21229326).
In vitro catalyzes 'Lys-11'-linked polyubiquitination. UBE2W-catalyzed ubiquitination occurs also in the presence of inactive RING/U-box type E3s, i.e. lacking the active site cysteine residues to form thioester bonds with ubiquitin, or even in the absence of E3, albeit at a slower rate (PubMed:25436519).
Biological Process
Biological Process cellular response to misfolded protein Source:UniProtKB
Biological Process DNA repair Source:UniProtKB-KW
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:UniProtKB
Biological Process protein K11-linked ubiquitination Source:UniProtKB1 Publication
Biological Process protein monoubiquitination Source:UniProtKB2 Publications
Biological Process protein polyubiquitination Source:GO_Central1 Publication
Biological Process protein quality control for misfolded or incompletely synthesized proteins Source:UniProtKB
Cellular Location
Nucleus
In the nucleus, colocalizes with FANCL.
PTM
Autoubiquitinated at Met-1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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