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Mouse Anti-TRIM24 Recombinant Antibody (CBYJT-4718) (CBMAB-T4255-YJ)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBYJT-4718
Antibody Isotype
IgG1
Application
ELISA, WB, IHC

Basic Information

Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TRIM24
Introduction
TRIM24 mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. It localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. It is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region.
Entrez Gene ID
Human8805
Mouse21848
UniProt ID
HumanO15164
MouseQ64127
Alternative Names
Tripartite Motif Containing 24; RING-Type E3 Ubiquitin Transferase TIF1-Alpha; Transcriptional Intermediary Factor 1; E3 Ubiquitin-Protein Ligase TRIM24; RING Finger Protein 82; TIF1-Alpha; RNF82; TIF1A; TIF1; Transcription Intermediary Factor 1-Alpha
Function
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation (PubMed:24820418).
Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).
Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway (PubMed:32324863).
Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3 (PubMed:33724611).
Biological Process
Biological Process calcium ion homeostasis Source:Ensembl
Biological Process cellular response to estrogen stimulus Source:UniProtKB1 Publication
Biological Process epithelial cell proliferation Source:Ensembl
Biological Process negative regulation of DNA-templated transcription Source:Ensembl
Biological Process negative regulation of epithelial cell proliferation Source:Ensembl
Biological Process positive regulation of gene expression Source:Ensembl
Biological Process protein autophosphorylation Source:Ensembl
Biological Process protein catabolic process Source:UniProtKB1 Publication
Biological Process protein ubiquitination Source:UniProtKB1 Publication
Biological Process regulation of apoptotic process Source:UniProtKB1 Publication
Biological Process regulation of protein stability Source:UniProtKB1 Publication
Biological Process regulation of signal transduction by p53 class mediator Source:Ensembl
Biological Process regulation of vitamin D receptor signaling pathway Source:Ensembl
Biological Process transcription by RNA polymerase II Source:ProtInc1 Publication
Cellular Location
Nucleus
Cytoplasm
Mitochondrion
Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. Translocated from nucleus to mitochondria to mediate antiviral immunity (PubMed:32324863).
Involvement in disease
A chromosomal aberration involving TRIM24/TIF1 is found in papillary thyroid carcinomas (PTCs). Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
PTM
Phosphorylated at Ser-768 by ATM kinase induces ubiquitination and degradation during DNA damage.
Sumoylated.
Undergoes ubiquitination-mediated degradation in response to DNA damage.
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For research use only. Not intended for any clinical use.

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