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Mouse Anti-TOP1 Recombinant Antibody (1291CT875.142.166) (CBMAB-T3645-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1291CT875.142.166
Antibody Isotype
IgG1, κ
Application
ELISA, WB, FC

Basic Information

Immunogen
Recombinant Protein
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Preservative
0.09% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TOP1
Introduction
TOP1 is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. TOP1 catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA.
Entrez Gene ID
7150
UniProt ID
P11387
Alternative Names
DNA Topoisomerase I; Topoisomerase (DNA) I; EC 5.99.1.2; Type I DNA Topoisomerase; DNA Topoisomerase 1; TOPI
Function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
Biological Process
Biological Process chromatin remodeling Source:UniProtKB1 Publication
Biological Process chromosome segregation Source:GO_Central1 Publication
Biological Process circadian regulation of gene expression Source:UniProtKB1 Publication
Biological Process circadian rhythm Source:UniProtKB1 Publication
Biological Process DNA replication Source:GO_Central1 Publication
Biological Process DNA topological change Source:UniProtKB1 Publication
Biological Process embryonic cleavage Source:Ensembl
Biological Process peptidyl-serine phosphorylation Source:CAFA1 Publication
Biological Process phosphorylation Source:UniProtKB1 Publication
Biological Process programmed cell death Source:UniProtKB1 Publication
Biological Process response to xenobiotic stimulus Source:UniProtKB1 Publication
Cellular Location
Nucleus, nucleolus
Nucleus, nucleoplasm
Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli.
Involvement in disease
A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
PTM
Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.
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For research use only. Not intended for any clinical use.

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