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Mouse Anti-TK1 Recombinant Antibody (CBYJT-3265) (CBMAB-T2550-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-3265
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
thymidine kinase 1
Introduction
TK1 is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. TK1, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.
Entrez Gene ID
7083
UniProt ID
P04183
Alternative Names
Thymidine Kinase 1; EC 2.7.1.21; Thymidine Kinase 1 Soluble Isoform; Thymidine Kinase 1, Soluble; Thymidine Kinase, Cytosolic; TK2
Function
Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed:9575153).
Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed:22385435).
Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed:22385435).
Biological Process
Biological Process DNA biosynthetic processSource:UniProtKB-KW
Biological Process nucleobase-containing compound metabolic processSource:ProtInc1 Publication
Biological Process phosphorylationSource:UniProtKB-KW
Biological Process protein homotetramerizationSource:UniProtKB1 Publication
Biological Process thymidine metabolic processSource:UniProtKB1 Publication
Cellular Location
Cytoplasm
PTM
Phosphorylated on Ser-13 in mitosis. Phosphorylation of Ser-13 by CDK1 during mitosis reduces homotetramerization and catalytic efficiency when DNA replication is complete and intracellular TK1 is still present at a high level (PubMed:9575153, PubMed:14697231).
Polyubiquitinated. Postmitosis, ubiquitination leads to proteasomal degradation. The KEN box sequence located at the C-terminal region targets for degradation by the anaphase promoting complex (APC/C) activated and rate-limited by FZR1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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