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Rabbit Anti-TDG Recombinant Antibody (CBYJT-2433) (CBMAB-T1579-YJ)


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Summary

Host Animal
Rabbit
Specificity
Human, Rat, Mouse
Clone
CBYJT-2433
Antibody Isotype
IgG
Application
WB, FC

Basic Information

Immunogen
Corresponding to Human TDG.
Specificity
Human, Rat, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Thymine DNA Glycosylase
Introduction
TDG belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a key role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.
Entrez Gene ID
Human6996
Mouse21665
Rat114521
UniProt ID
HumanQ13569
MouseP56581
RatQ99NG8
Alternative Names
Thymine DNA Glycosylase; G/T Mismatch-Specific Thymine DNA Glycosylase; HTDG; Thymine-DNA Glycosylase; EC 3.2.2.29
Function
DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T>> C/T> T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Biological Process
Biological Process base-excision repairIDA:UniProtKB2 Publications
Biological Process base-excision repair, AP site formationIDA:CAFA1 Publication
Biological Process depyrimidinationTAS:Reactome
Biological Process DNA demethylationISS:UniProtKB
Biological Process mismatch repairIDA:UniProtKB1 Publication
Biological Process negative regulation of chromatin bindingIEA:Ensembl
Biological Process negative regulation of protein bindingIEA:Ensembl
Biological Process negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Biological Process oxidative DNA demethylationTAS:Reactome
Biological Process regulation of DNA N-glycosylase activityIMP:CAFA1 Publication
Biological Process regulation of embryonic developmentISS:UniProtKB
Biological Process regulation of gene expression, epigeneticISS:UniProtKB
Cellular Location
Nucleus
PTM
Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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