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Mouse Anti-SPP1 Recombinant Antibody (X1) (CBMAB-O0867-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
X1
Antibody Isotype
IgG2b, Κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Recombinant corresponding to human SPP1
Specificity
Human
Antibody Isotype
IgG2b, Κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Secreted Phosphoprotein 1
Introduction
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
6696
UniProt ID
P10451
Alternative Names
Secreted Phosphoprotein 1; Early T-Lymphocyte Activation 1; Urinary Stone Protein; Nephropontin; Osteopontin; Uropontin; BNSP; OPN; Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early T-Lymphocyte Activation 1)
Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Biological Process
Biological Process androgen catabolic processManual Assertion Based On ExperimentIDA:CAFA
Biological Process biomineral tissue developmentIEA:UniProtKB-KW
Biological Process cell adhesionManual Assertion Based On ExperimentIDA:ARUK-UCL
Biological Process cellular response to testosterone stimulusManual Assertion Based On ExperimentIDA:CAFA
Biological Process decidualizationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process embryo implantationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process inflammatory responseIEA:Ensembl
Biological Process negative regulation of collateral sprouting of intact axon in response to injuryIEA:Ensembl
Biological Process osteoblast differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of bone resorptionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:CAFA
Biological Process positive regulation of estradiol secretionManual Assertion Based On ExperimentIDA:CAFA
Biological Process response to macrophage colony-stimulating factorIEA:Ensembl
Biological Process response to steroid hormoneIEA:Ensembl
Biological Process response to vitamin DManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular Location
Secreted
PTM
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties.
Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039).
The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity).
O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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