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Mouse Anti-SPP1 Recombinant Antibody (1D7) (CBMAB-A8552-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
1D7
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
SPP1 (AAH07016.1, 1 a.a. ~ 300 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Secreted Phosphoprotein 1
Entrez Gene ID
Human6696
Mouse20750
UniProt ID
HumanP10451
MouseP10923
Alternative Names
BNSP; BSPI; ETA-1; MGC110940; OPN
Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Biological Process
Biological Process androgen catabolic processManual Assertion Based On ExperimentIDA:CAFA
Biological Process biomineral tissue developmentIEA:UniProtKB-KW
Biological Process cell adhesionManual Assertion Based On ExperimentIDA:ARUK-UCL
Biological Process cellular response to testosterone stimulusManual Assertion Based On ExperimentIDA:CAFA
Biological Process decidualizationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process embryo implantationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process inflammatory responseIEA:Ensembl
Biological Process negative regulation of collateral sprouting of intact axon in response to injuryIEA:Ensembl
Biological Process osteoblast differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of bone resorptionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:CAFA
Biological Process positive regulation of estradiol secretionManual Assertion Based On ExperimentIDA:CAFA
Biological Process response to macrophage colony-stimulating factorIEA:Ensembl
Biological Process response to steroid hormoneIEA:Ensembl
Biological Process response to vitamin DManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular Location
Secreted
PTM
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties.
Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039).
The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity).
O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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