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Mouse Anti-SP100 Recombinant Antibody (1G6) (CBMAB-A8493-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1G6
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
SP100 (NP_003104, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
SP100 Nuclear Antigen
Entrez Gene ID
6672
UniProt ID
P23497
Alternative Names
DKFZp686E07254; FLJ00340; FLJ34579
Function
Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation.
Biological Process
Biological Process DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediatorManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process interferon-gamma-mediated signaling pathway1 PublicationIC:BHF-UCL
Biological Process maintenance of protein locationManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process negative regulation of DNA bindingManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process negative regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process negative regulation of DNA-templated transcriptionIDA:BHF-UCL
Biological Process negative regulation of endothelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of protein export from nucleusManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process negative regulation of viral transcriptionIDA:BHF-UCL
Biological Process positive regulation of DNA-binding transcription factor activityIDA:BHF-UCL
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process positive regulation of transcription by RNA polymerase IIIDA:BHF-UCL
Biological Process regulation of angiogenesisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of extrinsic apoptotic signaling pathway via death domain receptorsManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of Fas signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process response to cytokineIDA:BHF-UCL
Biological Process response to interferon-gammaManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process response to retinoic acidIDA:BHF-UCL
Biological Process response to type I interferonManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process retinoic acid receptor signaling pathwayIC:BHF-UCL
Biological Process telomere maintenanceManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process type I interferon signaling pathway1 PublicationIC:BHF-UCL
Cellular Location
Nucleus
Nucleus, PML body
Cytoplasm
Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon-dependent. Accumulates in the cytoplasm upon FAS activation.
Isoform Sp100-C
Nucleus
Forms a reticulate or track-like nuclear pattern with denser concentrations at the nuclear lamina and surrounding the nucleoli, a pattern reminiscent of heterochromatin-rich regions according to PubMed:11313457.
PTM
Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting.
Sumoylated. Sumoylated with SUMO1. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting. Sumoylation may stabilize the interaction with CBX5.
(Microbial infection) Immediate early protein IE1 of human cytomegalovirus (HHV-5) interferes with the sumoylation of SP100.
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For research use only. Not intended for any clinical use.

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