Rabbit Anti-SIRT2 Recombinant Antibody (CBXS-5401) (CBMAB-S2613-CQ)

Rabbit

Human, Mouse, Rat, Monkey

CBXS-5401

WB

Human, Mouse, Rat, Monkey

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

SIRT2

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene.

Sirtuin 2; Regulatory Protein SIR2 Homolog 2; SIR2-Like Protein 2; SIR2L2; SIR2L; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 2 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 2; NAD-Dependent Protein Deacetylase Sirtuin-2;

NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and alpha-tubulin as well as many other proteins such as key transcription factors (PubMed:24177535, PubMed:12620231, PubMed:16648462, PubMed:18249187, PubMed:18332217, PubMed:18995842, PubMed:20587414, PubMed:21081649, PubMed:20543840, PubMed:22014574, PubMed:21726808, PubMed:21949390, PubMed:22771473, PubMed:23468428, PubMed:23908241, PubMed:24940000, PubMed:24769394, PubMed:24681946).
Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy (PubMed:24177535, PubMed:12620231, PubMed:16648462, PubMed:18249187, PubMed:18332217, PubMed:18995842, PubMed:20587414, PubMed:21081649, PubMed:20543840, PubMed:22014574, PubMed:21726808, PubMed:21949390, PubMed:22771473, PubMed:23468428, PubMed:23908241, PubMed:24940000, PubMed:24769394, PubMed:24681946).
Plays a major role in the control of cell cycle progression and genomic stability (PubMed:12697818, PubMed:17488717, PubMed:16909107, PubMed:17726514, PubMed:19282667, PubMed:23468428).
Functions in the antephase checkpoint preventing precocious mitotic entry in response to microtubule stress agents, and hence allowing proper inheritance of chromosomes (PubMed:12697818, PubMed:17488717, PubMed:16909107, PubMed:17726514, PubMed:19282667, PubMed:23468428).
Positively regulates the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex activity by deacetylating CDC20 and FZR1, then allowing progression through mitosis (PubMed:22014574).
Associates both with chromatin at transcriptional start sites (TSSs) and enhancers of active genes (PubMed:23468428).
Plays a role in cell cycle and chromatin compaction through epigenetic modulation of the regulation of histone H4 'Lys-20' methylation (H4K20me1) during early mitosis (PubMed:23468428).
Specifically deacetylates histone H4 at 'Lys-16' (H4K16ac) between the G2/M transition and metaphase enabling H4K20me1 deposition by KMT5A leading to ulterior levels of H4K20me2 and H4K20me3 deposition throughout cell cycle, and mitotic S-phase progression (PubMed:23468428).
Deacetylates KMT5A modulating KMT5A chromatin localization during the mitotic stress response (PubMed:23468428).
Deacetylates also histone H3 at 'Lys-57' (H3K56ac) during the mitotic G2/M transition (PubMed:20587414).
Upon bacterium Listeria monocytogenes infection, deacetylates 'Lys-18' of histone H3 in a receptor tyrosine kinase MET- and PI3K/Akt-dependent manner, thereby inhibiting transcriptional activity and promoting late stages of listeria infection (PubMed:23908241).
During oocyte meiosis progression, may deacetylate histone H4 at 'Lys-16' (H4K16ac) and alpha-tubulin, regulating spindle assembly and chromosome alignment by influencing microtubule dynamics and kinetochore function (PubMed:24940000).
Deacetylates histone H4 at 'Lys-16' (H4K16ac) at the VEGFA promoter and thereby contributes to regulate expression of VEGFA, a key regulator of angiogenesis (PubMed:24940000).
Deacetylates alpha-tubulin at 'Lys-40' and hence controls neuronal motility, oligodendroglial cell arbor projection processes and proliferation of non-neuronal cells (PubMed:18332217, PubMed:18995842).
Phosphorylation at Ser-368 by a G1/S-specific cyclin E-CDK2 complex inactivates SIRT2-mediated alpha-tubulin deacetylation, negatively regulating cell adhesion, cell migration and neurite outgrowth during neuronal differentiation (PubMed:17488717).
Deacetylates PARD3 and participates in the regulation of Schwann cell peripheral myelination formation during early postnatal development and during postinjury remyelination (PubMed:21949390).
Involved in several cellular metabolic pathways (PubMed:20543840, PubMed:21726808, PubMed:24769394).
Plays a role in the regulation of blood glucose homeostasis by deacetylating and stabilizing phosphoenolpyruvate carboxykinase PCK1 activity in response to low nutrient availability (PubMed:21726808).
Acts as a key regulator in the pentose phosphate pathway (PPP) by deacetylating and activating the glucose-6-phosphate G6PD enzyme, and therefore, stimulates the production of cytosolic NADPH to counteract oxidative damage (PubMed:24769394).
Maintains energy homeostasis in response to nutrient deprivation as well as energy expenditure by inhibiting adipogenesis and promoting lipolysis (PubMed:20543840).
Attenuates adipocyte differentiation by deacetylating and promoting FOXO1 interaction to PPARG and subsequent repression of PPARG-dependent transcriptional activity (PubMed:20543840).
Plays a role in the regulation of lysosome-mediated degradation of protein aggregates by autophagy in neuronal cells (PubMed:20543840).
Deacetylates FOXO1 in response to oxidative stress or serum deprivation, thereby negatively regulating FOXO1-mediated autophagy (PubMed:20543840).
Deacetylates a broad range of transcription factors and co-regulators regulating target gene expression. Deacetylates transcriptional factor FOXO3 stimulating the ubiquitin ligase SCF(SKP2)-mediated FOXO3 ubiquitination and degradation (By similarity).
Deacetylates HIF1A and therefore promotes HIF1A degradation and inhibition of HIF1A transcriptional activity in tumor cells in response to hypoxia (PubMed:24681946).
Deacetylates RELA in the cytoplasm inhibiting NF-kappaB-dependent transcription activation upon TNF-alpha stimulation (PubMed:21081649).
Inhibits transcriptional activation by deacetylating p53/TP53 and EP300 (PubMed:18249187, PubMed:18995842).
Deacetylates also EIF5A (PubMed:22771473).
Functions as a negative regulator on oxidative stress-tolerance in response to anoxia-reoxygenation conditions (PubMed:24769394).
Plays a role as tumor suppressor (PubMed:22014574).
In addition to protein deacetylase activity, also has activity toward long-chain fatty acyl groups and mediates protein-lysine demyristoylation and depalmitoylation of target proteins, such as ARF6 and KRAS, thereby regulating their association with membranes (PubMed:25704306, PubMed:29239724, PubMed:32103017).
Isoform 1
Deacetylates EP300, alpha-tubulin and histone H3 and H4.
Isoform 2
Deacetylates EP300, alpha-tubulin and histone H3 and H4.
Isoform 5
Lacks deacetylation activity, at least toward known SIRT2 targets.

Biological Process autophagyIEA:UniProtKB-KW
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process cellular lipid catabolic processISS:UniProtKB
Biological Process cellular response to caloric restrictionISS:UniProtKB
Biological Process cellular response to epinephrine stimulusISS:UniProtKB
Biological Process cellular response to hepatocyte growth factor stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to hypoxiaManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to molecule of bacterial originManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to oxidative stressISS:UniProtKB
Biological Process hepatocyte growth factor receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process heterochromatin assembly1 PublicationNAS:UniProtKB
Biological Process histone deacetylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process histone H3 deacetylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process histone H4 deacetylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process innate immune responseIEA:UniProtKB-KW
Biological Process meiotic cell cycleIEA:UniProtKB-KW
Biological Process mitotic nuclear membrane reassemblyTAS:Reactome
Biological Process myelination in peripheral nervous systemISS:UniProtKB
Biological Process negative regulation of autophagyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of defense response to bacteriumManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of fat cell differentiationISS:UniProtKB
Biological Process negative regulation of NLRP3 inflammasome complex assemblyIEA:Ensembl
Biological Process negative regulation of oligodendrocyte progenitor proliferationISS:UniProtKB
Biological Process negative regulation of peptidyl-threonine phosphorylationISS:UniProtKB
Biological Process negative regulation of protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of reactive oxygen species metabolic processISS:UniProtKB
Biological Process negative regulation of striated muscle tissue developmentManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of transcription from RNA polymerase II promoter in response to hypoxiaManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process NLRP3 inflammasome complex assemblyIEA:Ensembl
Biological Process peptidyl-lysine deacetylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process phosphatidylinositol 3-kinase signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of attachment of spindle microtubules to kinetochoreISS:UniProtKB
Biological Process positive regulation of cell divisionISS:UniProtKB
Biological Process positive regulation of DNA bindingISS:UniProtKB
Biological Process positive regulation of execution phase of apoptosisISS:UniProtKB
Biological Process positive regulation of meiotic nuclear divisionISS:UniProtKB
Biological Process positive regulation of oocyte maturationISS:UniProtKB
Biological Process positive regulation of proteasomal ubiquitin-dependent protein catabolic processISS:UniProtKB
Biological Process positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxiaManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIISS:UniProtKB
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein ADP-ribosylationManual Assertion Based On ExperimentIDA:GO_Central
Biological Process protein deacetylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein kinase B signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process rDNA heterochromatin assembly1 PublicationNAS:UniProtKB
Biological Process regulation of cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of exit from mitosis1 PublicationNAS:UniProtKB
Biological Process regulation of myelinationISS:UniProtKB
Biological Process regulation of phosphorylation1 PublicationNAS:UniProtKB
Biological Process response to redox state1 PublicationNAS:UniProtKB
Biological Process substantia nigra developmentManual Assertion Based On ExperimentHEP:UniProtKB
Biological Process subtelomeric heterochromatin assembly1 PublicationNAS:UniProtKB
Biological Process tubulin deacetylationManual Assertion Based On ExperimentIDA:UniProtKB

Nucleus
Cytoplasm, perinuclear region
Cytoplasm
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Cytoplasm, cytoskeleton, spindle
Midbody
Chromosome
Perikaryon
Cell projection
Cell projection, growth cone
Myelin membrane
Localizes in the cytoplasm during most of the cell cycle except in the G2/M transition and during mitosis, where it is localized in association with chromatin and induces deacetylation of histone at 'Lys-16' (H4K16ac) (PubMed:17726514, PubMed:23468428).
Colocalizes with KMT5A at mitotic foci (PubMed:23468428).
Colocalizes with CDK1 at centrosome during prophase and splindle fibers during metaphase (PubMed:17488717).
Colocalizes with Aurora kinase AURKA at centrosome during early prophase and in the centrioles and growing mitotic spindle throughout metaphase (PubMed:17488717).
Colocalizes with Aurora kinase AURKB during cytokinesis with the midbody (PubMed:17488717).
Colocalizes with microtubules (PubMed:12620231).
Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins (By similarity).
Shuttles between the cytoplasm and the nucleus through the CRM1 export pathway (PubMed:17726514).
Colocalizes with EP300 in the nucleus (PubMed:24177535).
Translocates to the nucleus and chromatin upon bacterium Listeria monocytogenes infection in interphase cells (PubMed:23908241).
Deacetylates FOXO3 in the cytoplasm (By similarity).
Colocalizes with PLP1 in internodal regions, at paranodal axoglial junction and Schmidt-Lanterman incisures of myelin sheat (By similarity).
Colocalizes with CDK5R1 in the perikaryon, neurites and growth cone of hippocampal neurons (By similarity).
Colocalizes with alpha-tubulin in neuronal growth cone (By similarity).
Localizes in the cytoplasm and nucleus of germinal vesicle (GV) stage oocytes (By similarity).
Colocalizes with alpha-tubulin on the meiotic spindle as the oocytes enter into metaphase, and also during meiotic anaphase and telophase, especially with the midbody (By similarity).
Colocalizes with PARD3 in internodal region of axons (By similarity).
Colocalizes with acetylated alpha-tubulin in cell projection processes during primary oligodendrocyte precursor (OLP) differentiation (By similarity).
Isoform 1
Cytoplasm
Nucleus
Predominantly localized in the cytoplasmic.
Isoform 2
Cytoplasm
Nucleus
Predominantly localized in the cytoplasmic.
Isoform 5
Cytoplasm
Nucleus
Predominantly localized in the nucleus.

Phosphorylated at phosphoserine and phosphothreonine. Phosphorylated at Ser-368 by a mitotic kinase CDK1/cyclin B at the G2/M transition; phosphorylation regulates the delay in cell-cycle progression. Phosphorylated at Ser-368 by a mitotic kinase G1/S-specific cyclin E/Cdk2 complex; phosphorylation inactivates SIRT2-mediated alpha-tubulin deacetylation and thereby negatively regulates cell adhesion, cell migration and neurite outgrowth during neuronal differentiation. Phosphorylated by cyclin A/Cdk2 and p35-Cdk5 complexes and to a lesser extent by the cyclin D3/Cdk4 and cyclin B/Cdk1, in vitro. Dephosphorylated at Ser-368 by CDC14A and CDC14B around early anaphase.
Acetylated by EP300; acetylation leads both to the decreased of SIRT2-mediated alpha-tubulin deacetylase activity and SIRT2-mediated down-regulation of TP53 transcriptional activity.
Ubiquitinated.