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Rabbit Anti-SET Recombinant Antibody (CBXS-1339) (CBMAB-S4197-CQ)


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Summary

Host Animal
Rabbit
Specificity
Mouse, Rat, Human
Clone
CBXS-1339
Antibody Isotype
IgG
Application
WB, IP, IHC-P, FC, IF

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
SET
Introduction
The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
Human6418
Mouse56086
Rat120101889
UniProt ID
HumanQ01105
MouseQ9EQU5
RatQ63945
Alternative Names
SET Nuclear Proto-Oncogene; Template-Activating Factor-I, Chromatin Remodelling Factor; SET Translocation (Myeloid Leukemia-Associated); Inhibitor Of Granzyme A-Activated DNase; Protein Phosphatase Type 2A Inhibitor; Phosphatase 2A Inhibitor I2PP2A; HLA-DR-Associated Protein II; PHAPII; IGAAD; TAF-I;
Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Biological Process
Biological Process DNA replicationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process negative regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of histone acetylationManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process negative regulation of neuron apoptotic processManual Assertion Based On ExperimentIGI:MGI
Biological Process nucleosome assemblyManual Assertion Based On ExperimentTAS:ProtInc
Biological Process nucleosome disassemblyManual Assertion Based On ExperimentTAS:UniProtKB
Cellular Location
Cytoplasm, cytosol
Endoplasmic reticulum
Nucleus, nucleoplasm
In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. The SET complex is associated with the endoplasmic reticulum. Following CTL attack and cleavage by GZMA, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium.
Involvement in disease
Intellectual developmental disorder, autosomal dominant 58 (MRD58):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRD58 patients show delayed development, intellectual disability, language delay and speech impairment. Some patients have motor delay or incoordination, and minor dysmorphic features.
PTM
Isoform 2 is phosphorylated on Ser-15 and Ser-24.
Isoform 2 is acetylated on Lys-11.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated (By similarity).
Isoform 2
Cleaved after Lys-176 by GZMA. The cleavage inhibits its nucleosome assembly activity and disrupts the inhibition on NME1.
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For research use only. Not intended for any clinical use.

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