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Mouse Anti-ROBO3 Recombinant Antibody (2H1) (CBMAB-R3100-CN)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2H1
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Full length recombinant corresponding to aa. 1-148 from human ROBO3 (H08623) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2

Target

Full Name
roundabout, axon guidance receptor, homolog 3 (Drosophila)
Introduction
This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]
Entrez Gene ID
64221
UniProt ID
Q96MS0
Alternative Names
Roundabout Guidance Receptor 3; Roundabout-Like Protein 3; Roundabout, Axon Guidance Receptor, Homolog 3 (Drosophila); Roundabout (Axon Guidance Receptor, Drosophila) Homolog 3; Horizontal Gaze Palsy With Progressive Scoliosis; Roundabout, Axon Guidance Receptor, Homolog 3; Retinoblastoma Inhibiting Gene 1;
Function
Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube (By similarity).
In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity).
Required for hindbrain axon midline crossing (PubMed:15105459).
Biological Process
Biological Process axon guidanceISS:UniProtKB
Biological Process axon midline choice point recognitionISS:UniProtKB
Biological Process dendrite self-avoidanceManual Assertion Based On ExperimentIBA:GO_Central
Biological Process homophilic cell adhesion via plasma membrane adhesion moleculesManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Membrane
Involvement in disease
Gaze palsy, familial horizontal, with progressive scoliosis, 1 (HGPPS1):
An autosomal recessive neurologic disorder characterized by eye movement abnormalities apparent from birth, childhood-onset progressive scoliosis, distinctive brainstem malformation and defective crossing of some brainstem neuronal pathways.
Topology
Extracellular: 21-891
Helical: 892-912
Cytoplasmic: 913-1386
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For research use only. Not intended for any clinical use.

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