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Rabbit Anti-RNF20 Recombinant Antibody (10H23L1) (CBMAB-R3000-CN)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
10H23L1
Antibody Isotype
IgG
Application
ICC, IF, WB

Basic Information

Immunogen
Protein corresponding to Human RNF20 (aa. 96-433)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.2
Preservative
0.09% Sodium azide
Concentration
0.5 mg/mL

Target

Full Name
RNF20
Introduction
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]
Entrez Gene ID
Human56254
Mouse109331
Rat313216
UniProt ID
HumanQ5VTR2
MouseQ5DTM8
RatD3ZYQ9
Alternative Names
Ring Finger Protein 20; RING-Type E3 Ubiquitin Transferase BRE1A; RING Finger Protein 20; BRE1A; HBRE1; BRE1 E3 Ubiquitin Ligase Homolog (S. Cerevisiae); E3 Ubiquitin-Protein Ligase BRE1A;
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Biological Process
Biological Process chromatin organizationIEA:UniProtKB-KW
Biological Process histone H2B conserved C-terminal lysine ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process histone H2B ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process histone monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of histone methylationIEA:Ensembl
Biological Process positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

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