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Rabbit Anti-PHF8 Recombinant Antibody (E6K3Y) (CBMAB-CP1951-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
E6K3Y
Antibody Isotype
IgG
Application
WB, IP, CIP, CIP-seq

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with recombinant protein containing Phe579 of human PHF8 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
PHF8
Introduction
The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
Entrez Gene ID
Human23133
Mouse320595
Rat317425
Monkey703633
UniProt ID
HumanQ9UPP1
MouseQ80TJ7
RatD4AD31
MonkeyH9Z1P6
Alternative Names
PHD Finger Protein 8; Jumonji C Domain-Containing Histone Demethylase 1F; EC 1.14.11.27; ZNF422; Histone Lysine Demethylase PHF8; KIAA1111; JHDM1F; MRXSSD; KDM7B;
Function
Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
Biological Process
Brain developmentISS:UniProtKB
Chromatin organizationIEA:UniProtKB-KW
G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-K27 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K36 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H4-K20 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of ribosomal DNA heterochromatin assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Protein demethylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Nucleus, nucleolus
Recruited to H3K4me3 sites on chromatin during interphase (PubMed:20622854).
Dissociates from chromatin when cells enter mitosis (PubMed:20622854).
Involvement in disease
Intellectual developmental disorder, X-linked, syndromic, Siderius type (MRXSSD):
A syndrome characterized by mild to borderline mental retardation with or without cleft lip/cleft palate.
PTM
Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.
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For research use only. Not intended for any clinical use.

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