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Mouse Anti-PCBP1 (AA 1-225) Recombinant Antibody (CBFYH-2699) (CBMAB-H3757-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-2699
Antibody Isotype
IgG1
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, PH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-225

Target

Full Name
Poly(RC) Binding Protein 1
Introduction
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Entrez Gene ID
5093
UniProt ID
Q15365
Alternative Names
Poly(RC) Binding Protein 1; Heterogeneous Nuclear Ribonucleoprotein E1; Nucleic Acid-Binding Protein SUB2.3; Poly(RC)-Binding Protein 1; Alpha-CP1; Heterogenous Nuclear Ribonucleoprotein E1; Heterogenous Nuclear Ribonucleoprotein X; Epididymis Secretory Protein Li 85
Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).
Biological Process
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Viral RNA genome replicationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
PTM
Phosphorylated; lowers poly(rC)-binding activity.
More Infomation

Wu, Y., Mou, J., Liu, Y., & Zheng, W. (2023). Association of LncRNA PCBP1-AS1 with cancer occurrence and development: A review. Medicine, 102(43), e35631.

Luo, Y., Zhang, Y., Pang, S., Min, J., Wang, T., Wu, D., ... & Ma, L. (2023). PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation. Molecular Carcinogenesis, 62(7), 907-919.

Zhang, B., Zhang, M., Shen, C., Liu, G., Zhang, F., Hou, J., & Yao, W. (2021). LncRNA PCBP1-AS1-mediated AR/AR-V7 deubiquitination enhances prostate cancer enzalutamide resistance. Cell Death & Disease, 12(10), 856.

Jadhav, S., Protchenko, O., Li, F., Baratz, E., Shakoury-Elizeh, M., Maschek, A., ... & Philpott, C. C. (2021). Mitochondrial dysfunction in mouse livers depleted of iron chaperone PCBP1. Free Radical Biology and Medicine, 175, 18-27.

Huang, S., Luo, K., Jiang, L., Zhang, X. D., Lv, Y. H., & Li, R. F. (2021). PCBP1 regulates the transcription and alternative splicing of metastasis‑related genes and pathways in hepatocellular carcinoma. Scientific reports, 11(1), 23356.

Liao, C. Y., Lei, C. Q., & Shu, H. B. (2021). PCBP1 modulates the innate immune response by facilitating the binding of cGAS to DNA. Cellular & Molecular Immunology, 18(10), 2334-2343.

Ansa-Addo, E. A., Huang, H. C., Riesenberg, B., Iamsawat, S., Borucki, D., Nelson, M. H., ... & Li, Z. (2020). RNA binding protein PCBP1 is an intracellular immune checkpoint for shaping T cell responses in cancer immunity. Science advances, 6(22), eaaz3865.

Ishii, T., Igawa, T., Hayakawa, H., Fujita, T., Sekiguchi, M., & Nakabeppu, Y. (2020). PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions. Journal of Biological Chemistry, 295(34), 12247-12261.

Luo, T., Gao, Y., Zhangyuan, G., Xu, X., Xue, C., Jin, L., ... & Qin, X. (2020). lncRNA PCBP1-AS1 aggravates the progression of hepatocellular carcinoma via regulating PCBP1/PRL-3/AKT pathway. Cancer Management and Research, 5395-5408.

Patel, S. J., Frey, A. G., Palenchar, D. J., Achar, S., Bullough, K. Z., Vashisht, A., ... & Philpott, C. C. (2019). A PCBP1–BolA2 chaperone complex delivers iron for cytosolic [2Fe–2S] cluster assembly. Nature chemical biology, 15(9), 872-881.

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For research use only. Not intended for any clinical use.

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