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Mouse Anti-ORAI1 Recombinant Antibody (CBXO-0408) (CBMAB-O0510-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXO-0408
Antibody Isotype
IgG2a
Application
ELISA, WB, IHC

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ORAI Calcium Release-Activated Calcium Modulator 1
Introduction
The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1).
Entrez Gene ID
84876
UniProt ID
Q96D31
Alternative Names
ORAI Calcium Release-Activated Calcium Modulator 1; Transmembrane Protein 142A; Calcium Release-Activated Calcium Modulator 1; Protein Orai-1; TMEM142A; CRACM1
Function
Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1 (PubMed:16582901, PubMed:16645049, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:19249086, PubMed:23307288, PubMed:24351972, PubMed:24591628, PubMed:28219928, PubMed:20354224, PubMed:26956484).
CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT (PubMed:16582901).
Plays a prominent role in Ca2+ influx at the basolateral membrane of mammary epithelial cells independently of the Ca2+ content of endoplasmic reticulum or Golgi stores. May mediate transepithelial transport of large quantities of Ca2+ for milk secretion.
Biological Process
Adaptive immune responseIEA:UniProtKB-KW
Calcium ion importIEA:Ensembl
Calcium ion transmembrane transportManual Assertion Based On ExperimentIDA:FlyBase
Mammary gland epithelium developmentIEA:Ensembl
Positive regulation of adenylate cyclase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of calcium ion transportManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of calcium ion transportManual Assertion Based On ExperimentIMP:UniProtKB
Store-operated calcium entryManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cell membrane
Basolateral cell membrane
Isoform beta is more mobile in the plasma membrane (PubMed:23307288).
Colocalizes with STIM1 at the cell membrane (PubMed:27185316).
Involvement in disease
Immunodeficiency 9 (IMD9):
An immune disorder characterized by recurrent infections, impaired activation and proliferative response of T-cells, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhidrosis and non-progressive myopathy may be observed.
Myopathy, tubular aggregate, 2 (TAM2):
A rare congenital myopathy characterized by regular arrays of membrane tubules on muscle biopsies without additional histopathological hallmarks. Tubular aggregates in muscle are structures of variable appearance consisting of an outer tubule containing either one or more microtubule-like structures or amorphous material. TAM2 patients have myopathy and pupillary abnormalities.
Topology
Cytoplasmic: 1-87
Helical: 88-105
Extracellular: 106-119
Helical: 120-140
Cytoplasmic: 141-173
Helical: 174-194
Extracellular: 195-234
Helical: 235-255
Cytoplasmic: 256-301
PTM
N-glycosylated (PubMed:19249086, PubMed:26956484).
N-glycosylation inhibits channel activity in T cells (PubMed:26956484).
Ubiquitinated.
Cys-195 is oxidated, leading to inactivation of channel activity.
More Infomation

Novakovic, M. M., Korshunov, K. S., Grant, R. A., Martin, M. E., Valencia, H. A., Budinger, G. S., ... & Prakriya, M. (2023). Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels. Nature communications, 14(1), 5500.

Tsujikawa, S., DeMeulenaere, K. E., Centeno, M. V., Ghazisaeidi, S., Martin, M. E., Tapies, M. R., ... & Prakriya, M. (2023). Regulation of neuropathic pain by microglial Orai1 channels. Science Advances, 9(4), eade7002.

Szabó, V., Csákány-Papp, N., Görög, M., Madácsy, T., Varga, Á., Kiss, A., ... & Maléth, J. (2023). Orai1 calcium channel inhibition prevents progression of chronic pancreatitis. JCI insight, 8(13).

Jardin, I., Berna-Erro, A., Nieto-Felipe, J., Macias, A., Sanchez-Collado, J., Lopez, J. J., ... & Rosado, J. A. (2022). Similarities and differences between the Orai1 variants: Orai1α and Orai1β. International Journal of Molecular Sciences, 23(23), 14568.

Masson, B., Le Ribeuz, H., Sabourin, J., Laubry, L., Woodhouse, E., Foster, R., ... & Antigny, F. (2022). Orai1 inhibitors as potential treatments for pulmonary arterial hypertension. Circulation Research, 131(9), e102-e119.

Azimi, I., Stevenson, R. J., Zhang, X., Meizoso-Huesca, A., Xin, P., Johnson, M., ... & Monteith, G. R. (2020). A new selective pharmacological enhancer of the Orai1 Ca2+ channel reveals roles for Orai1 in smooth and skeletal muscle functions. ACS pharmacology & translational science, 3(1), 135-147.

Zhang, X., Xin, P., Yoast, R. E., Emrich, S. M., Johnson, M. T., Pathak, T., ... & Trebak, M. (2020). Distinct pharmacological profiles of ORAI1, ORAI2, and ORAI3 channels. Cell calcium, 91, 102281.

Yeung, P. S. W., Yamashita, M., & Prakriya, M. (2020). Molecular basis of allosteric Orai1 channel activation by STIM1. The Journal of physiology, 598(9), 1707-1723.

Lunz, V., Romanin, C., & Frischauf, I. (2019). STIM1 activation of Orai1. Cell calcium, 77, 29-38.

Zhang, X., Pathak, T., Yoast, R., Emrich, S., Xin, P., Nwokonko, R. M., ... & Trebak, M. (2019). A calcium/cAMP signaling loop at the ORAI1 mouth drives channel inactivation to shape NFAT induction. Nature communications, 10(1), 1971.

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For research use only. Not intended for any clinical use.

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