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Mouse Anti-NUMB Antibody (Cmnb-1) (CBMAB-1469CQ)


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Summary

Host Animal
Mouse
Specificity
Mouse, Human
Clone
Cmnb-1
Antibody Isotype
IgG2a, κ
Application
IF, WB

Basic Information

Immunogen
Recombinant GST/Numb (C-terminal 491 aa from long isoform) fusion protein
Specificity
Mouse, Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 163-653 (long isoform)

Target

Full Name
NUMB, Endocytic Adaptor Protein
Introduction
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Diseases associated with NUMB include Maxillary Sinus Adenoid Cystic Carcinoma and Tactile Agnosia. Among its related pathways are Signaling by NOTCH1 and Notch signaling pathway (KEGG).
Entrez Gene ID
Human8650
Mouse18222
UniProt ID
HumanP49757
MouseQ9QZS3
Alternative Names
Numb; NUMB endocytic adaptor protein; Nb
Function
Regulates clathrin-mediated receptor endocytosis (PubMed:18657069).
Plays a role in the process of neurogenesis (By similarity).
Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity).
Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity).
May also mediate local repair of brain ventricular wall damage (By similarity).
Biological Process
Adherens junction organizationIEA:Ensembl
AxonogenesisIEA:Ensembl
Lateral ventricle developmentISS:UniProtKB
Negative regulation of protein localization to plasma membraneManual Assertion Based On ExperimentIMP:UniProtKB
Neuroblast division in subventricular zoneISS:UniProtKB
Positive regulation of cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of neurogenesisISS:UniProtKB
Regulation of postsynaptic neurotransmitter receptor internalizationIEA:Ensembl
Cellular Location
Cell membrane
Endosome membrane
Localizes to perinuclear endosomes in an AAK1-dependent manner.
PTM
Phosphorylated on Ser-276 and Ser-295 by CaMK1.
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity).
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
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For research use only. Not intended for any clinical use.

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