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Mouse Anti-NT5E Recombinant Antibody (4D3-2C10) (CBMAB-A6154-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
4D3-2C10
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
NT5E (AAH15940.1, 28 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
5'-Nucleotidase Ecto
Introduction
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 oCcurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
Entrez Gene ID
4907
UniProt ID
P21589
Alternative Names
CD73; E5NT; NT; NT5; NTE; eN; eNT
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Biological Process
Adenosine biosynthetic processIEA:Ensembl
ADP catabolic processIEA:Ensembl
AMP catabolic processManual Assertion Based On ExperimentIBA:GO_Central
ATP metabolic processManual Assertion Based On ExperimentIDA:MGI
BiomineralizationManual Assertion Based On ExperimentIDA:MGI
Calcium ion homeostasisIEA:Ensembl
DNA metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Leukocyte cell-cell adhesionManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of inflammatory responseIEA:Ensembl
Response to ATPManual Assertion Based On ExperimentIDA:MGI
Response to inorganic substanceManual Assertion Based On ExperimentIDA:MGI
Cellular Location
Cell membrane
Involvement in disease
Calcification of joints and arteries (CALJA):
A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
More Infomation

Barge, S., Wu, A., Zhang, L., Robson, S. C., Olumi, A., Alper, S. L., ... & Yu, W. (2024). Role of ecto‐5′‐nucleotidase in bladder function. The FASEB Journal, 38(2), e23416.

Kordaß, T., Chao, T. Y., Osen, W., & Eichmüller, S. B. (2023). Novel microRNAs modulating ecto-5′-nucleotidase expression. Frontiers in Immunology, 14, 1199374.

Murakami, Y., Ando, M., Futamata, R., Horibe, T., Ueda, K., Kinoshita, M., & Kobayashi, T. (2022). Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes). Scientific Reports, 12(1), 18588.

da Silva, F. C., de Melo Neto, A. B., Martins, C. A., de Sousa Cardoso, T. C., de Souza Gomes, M., de Araújo, T. G., & Fürstenau, C. R. (2022). Is the regulation by miRNAs of NTPDase1 and ecto-5’-nucleotidase genes involved with the different profiles of breast cancer subtypes?. Purinergic Signalling, 18(1), 123-133.

Alcedo, K. P., Bowser, J. L., & Snider, N. T. (2021). The elegant complexity of mammalian ecto-5′-nucleotidase (CD73). Trends in cell biology, 31(10), 829-842.

Shali, S., Yu, J., Zhang, X., Wang, X., Jin, Y., Su, M., ... & Zhou, P. (2019). Ecto‐5′‐nucleotidase (CD73) is a potential target of hepatocellular carcinoma. Journal of cellular physiology, 234(7), 10248-10259.

Minor, M., Alcedo, K. P., Battaglia, R. A., & Snider, N. T. (2019). Cell type-and tissue-specific functions of ecto-5′-nucleotidase (CD73). American Journal of Physiology-Cell Physiology, 317(6), C1079-C1092.

Hu, S., Meng, F., Yin, X., Cao, C., & Zhang, G. (2019). NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer. Bioscience reports, 39(5), BSR20190101.

Bertoni, A. P. S., Bracco, P. A., de Campos, R. P., Lutz, B. S., Assis-Brasil, B. M., de Souza Meyer, E. L., ... & Wink, M. R. (2019). Activity of ecto-5′-nucleotidase (NT5E/CD73) is increased in papillary thyroid carcinoma and its expression is associated with metastatic lymph nodes. Molecular and cellular endocrinology, 479, 54-60.

Kobarfard, F., Noroozi, R., Beirami, A. D., Taheri, M., & Ghafouri-Fard, S. (2019). Dys-regulation of peripheral transcript levels of ecto-5’-nucleotidase in multiple sclerosis patients. Human Antibodies, 27(3), 161-165.

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For research use only. Not intended for any clinical use.

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