Mouse Anti-NSG1 Recombinant Antibody (1C3) (CBMAB-A2065-LY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Rat

Clone

1C3

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

D4S234E (AAH01745, 1 a.a. ~ 185 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human, Rat

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

neuron specific gene family member 1

Entrez Gene ID

Human	27065
Rat	25247

UniProt ID

Human	P42857
Rat	P02683

Alternative Names

D4S234; NEEP21; NSG1; P21

Function

Plays a role in the recycling mechanism in neurons of multiple receptors, including AMPAR, APP and L1CAM and acts at the level of early endosomes to promote sorting of receptors toward a recycling pathway. Regulates sorting and recycling of GRIA2 through interaction with GRIP1 and then contributes to the regulation of synaptic transmission and plasticity by affecting the recycling and targeting of AMPA receptors to the synapse (By similarity).
Is required for faithful sorting of L1CAM to axons by facilitating trafficking from somatodendritic early endosome or the recycling endosome (By similarity).
In an other hand, induces apoptosis via the activation of CASP3 in response to DNA damage (PubMed:20599942, PubMed:20878061).

Biological Process

Amyloid precursor protein metabolic processISS:UniProtKB
Apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Clathrin coat assemblyManual Assertion Based On ExperimentIBA:GO_Central
Dopamine receptor signaling pathwayIEA:InterPro
Endosomal transportManual Assertion Based On ExperimentIBA:GO_Central
Neurotransmitter receptor cycleISS:UniProtKB
Neurotransmitter receptor transport, endosome to postsynaptic membraneIEA:Ensembl
Positive regulation of receptor recyclingIEA:Ensembl
Postsynaptic neurotransmitter receptor cycleISS:UniProtKB
Receptor recyclingISS:UniProtKB
Regulation of long-term synaptic potentiationISS:UniProtKB
Spontaneous synaptic transmissionISS:UniProtKB
Vesicle-mediated transport in synapseIEA:Ensembl

Cellular Location

Membrane
Golgi apparatus, trans-Golgi network membrane
Endosome membrane
Cell projection, dendrite
Early endosome membrane
Late endosome membrane
Lysosome lumen
Recycling endosome membrane
Cytoplasmic vesicle membrane
Golgi apparatus, Golgi stack membrane
Endosome, multivesicular body membrane
Endoplasmic reticulum membrane
Endocytosed from the cell surface, thus enters into early endosomes, trafficks to late endosomes and degradates in lysosomes (By similarity).
Endoplasmic reticulum targeting is essential for apoptosis (PubMed:20599942).
Found in both stationary and motile endosomes. A previous study supports a type I membrane protein topology (By similarity).

Topology

Cytoplasmic: 1-82
Helical: 83-103
Lumenal: 104-185

More Infomation

References

Qi, L., Sun, C., Sun, S., Li, A., Hu, Q., Liu, Y., & Zhang, Y. (2023). Phosphatidylinositol (3, 5)-bisphosphate machinery regulates neurite thickness through neuron-specific endosomal protein NSG1/NEEP21. Journal of Biological Chemistry, 299(1).

Overby, M., Serrano-Rodriguez, A., Dadras, S., Christiansen, A. K., Ozcelik, G., Lichtenthaler, S. F., ... & Müller, H. K. (2023). Neuron-specific gene NSG1 binds to and positively regulates sortilin ectodomain shedding via a metalloproteinase-dependent mechanism. Journal of Biological Chemistry, 299(12).

Tu, M., Yin, X., Zhuang, W., Lin, X., Xia, Y., Huang, Z., ... & Huang, Y. (2023). NSG1 promotes glycolytic metabolism to enhance Esophageal squamous cell carcinoma EMT process by upregulating TGF-β. Cell Death Discovery, 9(1), 391.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-NSG1 Recombinant Antibody (1D22)

Mouse Anti-NSG1 Recombinant Antibody (CBWJN-1034)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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