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Mouse Anti-NR3C2 (AA 1-670) Recombinant Antibody (CBFYM-3034) (CBMAB-M3230-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-3034
Antibody Isotype
IgG2b
Application
WB, IHC

Basic Information

Immunogen
E. coli-derived recombinant human Mineralocorticoid R/NR3C2, Met1-Leu670
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS
Concentration
LYOPH
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-670

Target

Full Name
Nr3c2
Introduction
This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
4306
UniProt ID
P08235
Alternative Names
Nuclear Receptor Subfamily 3 Group C Member 2; MLR; MCR; MR; Nuclear Receptor Subfamily 3, Group C, Member 2 Variant 3; Nuclear Receptor Subfamily 3, Group C, Member 2; Mineralocorticoid Receptor Delta
Function
Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
Biological Process
Intracellular steroid hormone receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIMP:ARUK-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus
Endoplasmic reticulum membrane
Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
Involvement in disease
Pseudohypoaldosteronism 1, autosomal dominant (PHA1A):
A salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. PHA1A is a mild form characterized by target organ defects confined to kidney. Patients may present with neonatal renal salt wasting with hyperkalaemic acidosis despite high aldosterone levels. These patients improve with age and usually become asymptomatic without treatment.
Early-onset hypertension with severe exacerbation in pregnancy (EOHSEP):
Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
PTM
Phosphorylated.
More Infomation

Amin, M., Syed, S., Wu, R., Postolache, T. T., & Gragnoli, C. (2023). Novel linkage and association of the mineralocorticoid receptor gene (NR3C2) with familial type 2 diabetes and depression and their comorbidity. Aspects of Molecular Medicine, 1, 100003.

Li, J., & Xu, Z. (2022). NR3C2 suppresses the proliferation, migration, invasion and angiogenesis of colon cancer cells by inhibiting the AKT/ERK signaling pathway. Molecular Medicine Reports, 25(4), 1-8.

Li, X., Yang, A., Wen, P., Yuan, Y., Xiao, Z., Shi, H., & Wang, R. (2022). Nuclear receptor subfamily 3 group c member 2 (NR3C2) is downregulated due to hypermethylation and plays a tumor-suppressive role in colon cancer. Molecular and Cellular Biochemistry, 477(11), 2669-2679.

Peng, Y., Xi, X., Li, J., Ni, J., Yang, H., Wen, C., & Wen, M. (2021). miR‐301b and NR3C2 co‐regulate cells malignant properties and have the potential to be independent prognostic factors in breast cancer. Journal of Biochemical and Molecular Toxicology, 35(2), e22650.

Lu, J., Hu, F., & Zhou, Y. (2021). NR3C2-related transcriptome profile and clinical outcome in invasive breast carcinoma. BioMed research international, 2021.

Fan, Y., Li, Y., Zhu, Y., Dai, G., Wu, D., Gao, Z., ... & Xu, D. (2021). miR-301b-3p regulates breast cancer cell proliferation, migration, and invasion by targeting NR3C2. Journal of Oncology, 2021.

Cukier, H. N., Griswold, A. J., Hofmann, N. K., Gomez, L., Whitehead, P. L., Abramson, R. K., ... & Pericak‐Vance, M. A. (2020). Three Brothers With Autism Carry a Stop‐Gain Mutation in the HPA‐Axis Gene NR3C2. Autism research, 13(4), 523-531.

Kumsta, R., Kliegel, D., Linden, M., DeRijk, R., & de Kloet, E. R. (2019). Genetic variation of the mineralocorticoid receptor gene (MR, NR3C2) is associated with a conceptual endophenotype of “CRF-hypoactivity”. Psychoneuroendocrinology, 105, 79-85.

Yang, C., Ma, X., Guan, G., Liu, H., Yang, Y., Niu, Q., ... & Zhuang, L. (2019). MicroRNA‐766 promotes cancer progression by targeting NR3C2 in hepatocellular carcinoma. The FASEB Journal, 33(1), 1456-1467.

Cai, J., Shangguan, S., Li, G., Cai, Y., Chen, Y., Ma, G., ... & Deng, Y. (2019). Knockdown of lncRNA Gm11974 protect against cerebral ischemic reperfusion through miR-766-3p/NR3C2 axis. Artificial cells, nanomedicine, and biotechnology, 47(1), 3847-3853.

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For research use only. Not intended for any clinical use.

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