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Rabbit Anti-NOS3 Recombinant Antibody (7H43) (CBMAB-N2997-WJ)

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Summary

Host Animal
Rabbit
Specificity
Cattle, Human
Clone
7H43
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Specificity
Cattle, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nitric Oxide Synthase 3
Introduction
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
Entrez Gene ID
Human4846
Cattle287024
UniProt ID
HumanP29474
CattleP29473
Alternative Names
Nitric Oxide Synthase 3; Nitric Oxide Synthase 3 (Endothelial Cell); Constitutive NOS; Endothelial NOS; EC 1.14.13.39; NOS Type III; EC-NOS; NOSIII; CNOS;
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832).
NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C
Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Biological Process
AngiogenesisIEA:Ensembl
Aortic valve morphogenesisISS:BHF-UCL
Arginine catabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Blood vessel diameter maintenanceISS:BHF-UCL
Blood vessel remodelingISS:BHF-UCL
Cell redox homeostasisTAS:Reactome
Endocardial cushion morphogenesisISS:BHF-UCL
Endothelial cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Homeostasis of number of cells within a tissueISS:BHF-UCL
In utero embryonic developmentIEA:Ensembl
Lipopolysaccharide-mediated signaling pathwayIEA:Ensembl
Lung developmentIEA:Ensembl
Mitochondrion organizationISS:BHF-UCL
Negative regulation of biomineral tissue developmentISS:BHF-UCL
Negative regulation of blood pressureManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of calcium ion transportIEA:Ensembl
Negative regulation of cell population proliferationISS:BHF-UCL
Negative regulation of extrinsic apoptotic signaling pathway via death domain receptorsISS:BHF-UCL
Negative regulation of hydrolase activityIEA:Ensembl
Negative regulation of muscle hyperplasiaISS:BHF-UCL
Negative regulation of platelet activation1 PublicationNAS:BHF-UCL
Negative regulation of potassium ion transportIEA:Ensembl
Negative regulation of smooth muscle cell proliferationIEA:Ensembl
Nitric oxide biosynthetic processManual Assertion Based On ExperimentIDA:BHF-UCL
Nitric oxide mediated signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Ovulation from ovarian follicleIEA:Ensembl
Positive regulation of angiogenesisManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of blood vessel endothelial cell migrationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of gene expressionBy SimilarityISS:BHF-UCL
Positive regulation of guanylate cyclase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process positive regulation of Notch signaling pathway1 PublicationIC:BHF-UCL
Pulmonary valve morphogenesisISS:BHF-UCL
Regulation of blood pressure1 PublicationNAS:BHF-UCL
Regulation of nervous system processISS:ARUK-UCL
Regulation of sodium ion transportIEA:Ensembl
Regulation of systemic arterial blood pressure by endothelinManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of the force of heart contraction by chemical signalIEA:Ensembl
Removal of superoxide radicalsManual Assertion Based On ExperimentIDA:BHF-UCL
Response to fluid shear stressManual Assertion Based On ExperimentIEP:BHF-UCL
Response to heat1 PublicationNAS:BHF-UCL
Response to hormoneManual Assertion Based On ExperimentIBA:GO_Central
Response to lipopolysaccharideManual Assertion Based On ExperimentIBA:GO_Central
Smooth muscle hyperplasiaISS:BHF-UCL
Vasodilation2 PublicationsNAS:BHF-UCL
Ventricular septum morphogenesisIEA:Ensembl
Cellular Location
Cell membrane
Membrane, caveola
Cytoplasm, cytoskeleton
Golgi apparatus
Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Involvement in disease
Variation Asp-298 in NOS3 may be associated with susceptibility to coronary spasm.
PTM
Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity).
Phosphorylation of Ser-114 by CDK5 reduces activity.
More Infomation

Wetzel, M. D., Stanley, K., Maity, S., Madesh, M., Bopassa, J. C., & Awad, A. S. (2021). Homoarginine ameliorates diabetic nephropathy independent of nitric oxide synthase‐3. Physiological Reports, 9(5), e14766.

Kanipakam, H., Sharma, K., Thinlas, T., Mohammad, G., & Pasha, M. Q. (2021). Structural and functional alterations of nitric oxide synthase 3 due to missense variants associate with high-altitude pulmonary edema through dynamic study. Journal of Biomolecular Structure and Dynamics, 39(1), 294-309.

Agúndez, J. A., García-Martín, E., Rodríguez, C., Benito-León, J., Millán-Pascual, J., Díaz-Sánchez, M., ... & Jiménez-Jiménez, F. J. (2020). Endothelial nitric oxide synthase (NOS3) rs2070744 polymorphism and risk for multiple sclerosis. Journal of Neural Transmission, 127, 1167-1175.

Garcia-Martin, E., Navarro-Munoz, S., Rodriguez, C., Serrador, M., Alonso-Navarro, H., Calleja, M., ... & Jiménez-Jiménez, F. J. (2020). Association between endothelial nitric oxide synthase (NOS3) rs2070744 and the risk for migraine. The pharmacogenomics journal, 20(3), 426-432.

Kondkar, A. A., Azad, T. A., Sultan, T., Osman, E. A., Almobarak, F. A., & Al-Obeidan, S. A. (2020). Association of endothelial nitric oxide synthase (NOS3) gene polymorphisms with primary open-angle glaucoma in a Saudi cohort. PLoS One, 15(1), e0227417.

Luo, Z., Jia, A., Lu, Z., Muhammad, I., Adenrele, A., & Song, Y. (2019). Associations of the NOS3 rs1799983 polymorphism with circulating nitric oxide and lipid levels: a systematic review and meta-analysis. Postgraduate Medical Journal, 95(1125), 361-371.

Teralı, K., & Ergören, M. Ç. (2019). The contribution of NOS3 variants to coronary artery disease: a combined genetic epidemiology and computational biochemistry perspective. International journal of biological macromolecules, 123, 494-499.

González-Castro, T. B., Genis-Mendoza, A. D., Tovilla-Zárate, C. A., Juárez-Rojop, I. E., López-Narvaez, M. L., Pérez-Hernández, N., ... & Martínez-Magaña, J. J. (2019). Association between polymorphisms of NOS1, NOS2 and NOS3 genes and suicide behavior: a systematic review and meta-analysis. Metabolic brain disease, 34, 967-977.

Aouf, S., Laribi, A., Gabbouj, S., Hassen, E., Bouaouinaa, N., Zakhama, A., & Harizi, H. (2019). Contribution of Nitric oxide synthase 3 genetic variants to nasopharyngeal carcinoma risk and progression in a Tunisian population. European Archives of Oto-Rhino-Laryngology, 276, 1231-1239.

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For research use only. Not intended for any clinical use.

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