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Mouse Anti-NNT Recombinant Antibody (1D6) (CBMAB-N2848-WJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1D6
Antibody Isotype
IgG1, κ
Application
ELISA, IF, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nicotinamide Nucleotide Transhydrogenase
Introduction
This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016]
Entrez Gene ID
23530
UniProt ID
Q13423
Alternative Names
Nicotinamide Nucleotide Transhydrogenase; Pyridine Nucleotide Transhydrogenase; EC 1.6.1.2; NAD(P) Transhydrogenase, Mitochondrial; GCCD4;
Function
The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane (By similarity).
May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland (PubMed:22634753).
Biological Process
NADPH regenerationManual Assertion Based On ExperimentIBA:GO_Central
Proton transmembrane transportManual Assertion Based On ExperimentTAS:UniProtKB
Reactive oxygen species metabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Tricarboxylic acid cycleManual Assertion Based On ExperimentTAS:UniProtKB
Cellular Location
Mitochondrion inner membrane
Involvement in disease
Glucocorticoid deficiency 4 with or without mineralocorticoid deficiency (GCCD4):
A form of glucocorticoid deficiency, a rare autosomal recessive disorder characterized by resistance to ACTH action on the adrenal cortex, adrenal insufficiency and an inability of the adrenal cortex to produce cortisol. It usually presents in the neonatal period or in early childhood with episodes of hypoglycemia and other symptoms related to cortisol deficiency, including failure to thrive, recurrent illnesses or infections, convulsions, and shock. In a small number of patients hypoglycemia can be sufficiently severe and persistent that it leads to serious long-term neurological damage or death. The diagnosis is readily confirmed with a low plasma cortisol measurement in the presence of an elevated ACTH level, and normal aldosterone and plasma renin measurements.
More Infomation

Grayson, C., & Mailloux, R. J. (2023). Coenzyme Q10 and nicotinamide nucleotide transhydrogenase: Sentinels for mitochondrial hydrogen peroxide signaling. Free Radical Biology and Medicine.

Bodoni, A. F., Coeli-Lacchini, F. B., Gebenlian, J. L., Sobral, L. M., Garcia, C. B., Silva Jr, W. A., ... & Antonini, S. R. R. (2023). Nicotinamide Nucleotide Transhydrogenase Is Essential for Adrenal Steroidogenesis: Clinical and In Vitro Lessons. The Journal of Clinical Endocrinology & Metabolism, 108(6), 1464-1474.

Regan, T., Conway, R., & Bharath, L. P. (2022). Regulation of immune cell function by nicotinamide nucleotide transhydrogenase. American Journal of Physiology-Cell Physiology, 322(4), C666-C673.

Francisco, A., Figueira, T. R., & Castilho, R. F. (2022). Mitochondrial NAD (P)+ transhydrogenase: from molecular features to physiology and disease. Antioxidants & Redox Signaling, 36(13), 864-884.

Close, A. F., Chae, H., & Jonas, J. C. (2021). The lack of functional nicotinamide nucleotide transhydrogenase only moderately contributes to the impairment of glucose tolerance and glucose-stimulated insulin secretion in C57BL/6J vs C57BL/6N mice. Diabetologia, 64(11), 2550-2561.

Duan, Z., Song, Y., Zou, X., Liu, S., Zhang, W., & Liu, L. (2020). Nicotinamide nucleotide transhydrogenase acts as a new prognosis biomarker in hepatocellular carcinoma. International Journal of Clinical and Experimental Pathology, 13(5), 972.

Rao, K. S., Shen, X., Pardue, S., & Krzywanski, D. M. (2020). Nicotinamide nucleotide transhydrogenase (NNT) regulates mitochondrial ROS and endothelial dysfunction in response to angiotensin II. Redox Biology, 36, 101650.

Ward, N. P., Kang, Y. P., Falzone, A., Boyle, T. A., & DeNicola, G. M. (2020). Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function. Journal of Experimental Medicine, 217(6).

Kunath, A., Heiker, J. T., Kern, M., Kosacka, J., Flehmig, G., Stumvoll, M., ... & Klöting, N. (2020). Nicotinamide nucleotide transhydrogenase (nnt) is related to obesity in mice. Hormone and Metabolic Research, 52(12), 877-881.

McCambridge, G., Agrawal, M., Keady, A., Kern, P. A., Hasturk, H., Nikolajczyk, B. S., & Bharath, L. P. (2019). Saturated fatty acid activates T cell inflammation through a nicotinamide nucleotide transhydrogenase (NNT)-dependent mechanism. Biomolecules, 9(2), 79.

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For research use only. Not intended for any clinical use.

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