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Mouse Anti-NMI Recombinant Antibody (9D8) (CBMAB-N0430-WJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
9D8
Antibody Isotype
IgG2b, κ
Application
ELISA, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
N-Myc And STAT Interactor
Introduction
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]
Entrez Gene ID
9111
UniProt ID
Q13287
Alternative Names
N-Myc And STAT Interactor; N-Myc (And STAT) Interactor; N-Myc-Interactor; NMI;
Function
Acts as a signaling pathway regulator involved in innate immune system response (PubMed:9989503, PubMed:26342464, PubMed:29038465, PubMed:29350881).
In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis (PubMed:9989503, PubMed:29377960).
Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription (PubMed:9989503).
In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 (PubMed:10779520, PubMed:10950963).
In complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21 (PubMed:26342464).
In complex with IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881).
Negatively regulates virus-triggered type I interferon/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering with cellular antiviral responses (By similarity).
Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellular space during cell injury or pathogen invasion (PubMed:29038465).
Macrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines (PubMed:29038465).
Biological Process
Interferon-gamma-mediated signaling pathwayIEA:InterPro
Macrophage activation involved in immune responseManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of innate immune responseManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of interferon-alpha productionIEA:Ensembl
Negative regulation of interferon-beta productionIEA:Ensembl
Negative regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of inflammatory responseISS:UniProtKB
Positive regulation of innate immune responseISS:UniProtKB
Positive regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein K48-linked ubiquitinationIEA:Ensembl
Receptor signaling pathway via JAK-STATManual Assertion Based On ExperimentTAS:UniProtKB
Response to virusISS:UniProtKB
Toll-like receptor 4 signaling pathwayISS:UniProtKB
Transcription by RNA polymerase IIIEA:InterPro
Cellular Location
Cytoplasm
Nucleus
Secreted
Cytoplasmic NMI localizes in punctate granular structures (PubMed:9781816, PubMed:10950963).
Nuclear localization increased following IFN-alpha treatment (PubMed:9781816, PubMed:10950963).
Extracelullar following secretion by macrophage (PubMed:29038465).
PTM
Ubiquitinated. 'Lys-63'-linked ubiquitination by TRIM21 promotes interaction with IFI35 and inhibits virus-triggered type I IFN-beta production.
More Infomation

Hu, H., Li, B., Chen, H., Fan, G., Ye, Z., Ji, S., ... & Qin, Y. (2024). NMI promotes tumor progression and gemcitabine resistance in pancreatic cancer via STAT3‐IFIT3 axis. Molecular Carcinogenesis, 63(2), 195-208.

Feng, L., Li, W., Li, X., Li, X., Ran, Y., Yang, X., ... & Li, H. (2023). N‐MYC‐interacting protein enhances type II interferon signaling by inhibiting STAT1 sumoylation. The FASEB Journal, 37(12), e23281.

Li, L., Chen, S. N., Li, N., & Nie, P. (2022). Molecular characterization and transcriptional conservation of N-myc-interactor, Nmi, by type I and type II IFNs in mandarin fish Siniperca chuatsi. Developmental & Comparative Immunology, 130, 104354.

Zhang, W., Zhou, H., Cen, M., Ouyang, W., Chen, J., Xia, L., ... & Xu, F. (2022). N-myc and STAT interactor is a novel biomarker of severity in community-acquired pneumonia: a prospective study. Respiratory Research, 23(1), 1-11.

Banday, M. M., Kumar, A., Vestal, G., Sethi, J., Patel, K. N., O'Neill, E. B., ... & Sharma, N. S. (2021). N-myc-interactor mediates microbiome induced epithelial to mesenchymal transition and is associated with chronic lung allograft dysfunction. The Journal of Heart and Lung Transplantation, 40(6), 447-457.

Wu, S., Li, X., Chai, H., Feng, L., Li, W., & Li, H. (2021). Downregulation of N-myc interactor promotes cervical cancer cells growth by activating Stat3 signaling. Cell Biochemistry and Biophysics, 79, 103-111.

Ji, D., Feng, Y., Peng, W., Li, J., Gu, Q. O., Zhang, Z., ... & Sun, Y. (2020). NMI promotes cell proliferation through TGFβ/Smad pathway by upregulating STAT1 in colorectal cancer. Journal of Cellular Physiology, 235(1), 429-441.

Choi, J. E., Hyun, C. L., Jin, M. S., Lee, K. M., Moon, J. H., & Ryu, H. S. (2020). Downregulation of N-myc and STAT interactor protein predicts aggressive tumor behavior and poor prognosis in invasive ductal carcinoma. Journal of Breast Cancer, 23(1), 36-46.

Xia, J., Blank, M. L., Zhou, L. J., Wu, S. Z., Peng, H. J., & Boyle, J. P. (2020). Strain-specific disruption of interferon-stimulated N-myc and STAT interactor (NMI) function by Toxoplasma gondii type I ROP18 in human cells. Parasitology, 147(13), 1433-1442.

Xiong, L., Du, Y., Zhou, T., Du, B., Visalath, P., Lin, L., ... & Cai, W. (2019). N‐myc and STAT interactor correlates with severity and prognosis in acute‐on‐chronic liver failure of hepatitis B virus. Journal of Gastroenterology and Hepatology, 34(10), 1800-1808.

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For research use only. Not intended for any clinical use.

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