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Mouse Anti-NKX2-1 Recombinant Antibody (NX2.1/690) (CBMAB-T0531-YJ)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
NX2.1/690
Antibody Isotype
IgG1, κ
Application
FC, IHC-P, IF, CyTOF

Basic Information

Immunogen
Recombinant TTF-1 protein
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Homeobox protein Nkx-2.1
Introduction
NKX2.1 is a protein initially identified as a thyroid-specific transcription factor. NKX2.1 binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in NKX2.1 are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer.
Entrez Gene ID
Human7080
Mouse21869
Rat25628
UniProt ID
HumanP43699
MouseP50220
RatP23441
Alternative Names
BCH; BHC; NK-2; TEBP; TTF1; NKX2A; NMTC1; T/EBP; TITF1; TTF-1; NKX2.1
Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
Biological Process
Brain developmentManual Assertion Based On ExperimentIMP:UniProtKB
Cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Epithelial tube branching involved in lung morphogenesisManual Assertion Based On ExperimentIEP:UniProtKB
Forebrain developmentManual Assertion Based On ExperimentIEP:UniProtKB
Globus pallidus developmentManual Assertion Based On ExperimentIMP:UniProtKB
Lung developmentManual Assertion Based On ExperimentIEP:UniProtKB
Negative regulation of cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of epithelial to mesenchymal transitionManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:MGI
Negative regulation of transcription, DNA-templatedISS:UniProtKB
Negative regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of circadian rhythmISS:UniProtKB
Positive regulation of gene expressionManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:ComplexPortal
Response to hormoneManual Assertion Based On ExperimentIEP:UniProtKB
Rhythmic processIEA:UniProtKB-KW
Thyroid gland developmentManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Involvement in disease
Chorea, hereditary benign (BHC):
A rare autosomal dominant movement disorder, defined by early onset in childhood, a stable or non-progressive course of chorea, and no mental deterioration. Chorea is characterized by involuntary, forcible, rapid, jerky movements that may be subtle or become confluent, markedly altering normal patterns of movement.
Choreoathetosis and congenital hypothyroidism with or without pulmonary dysfunction (CAHTP):
An autosomal dominant disorder that manifests in infancy with neurological disturbances, hypothyroidism, and respiratory problems. It is characterized by movement abnormalities beginning with muscular hypotonia followed by the development of chorea, athetosis, dystonia, ataxia, and dysarthria.
Thyroid cancer, non-medullary, 1 (NMTC1):
A form of non-medullary thyroid cancer (NMTC), a cancer characterized by tumors originating from the thyroid follicular cells. NMTCs represent approximately 95% of all cases of thyroid cancer and are classified into papillary, follicular, Hurthle cell, and anaplastic neoplasms.
PTM
Phosphorylated on serine residues by STK3/MST2.
More Infomation

Soreze, Y., Nathan, N., Jegard, J., Hervieux, E., Clermidi, P., Sileo, C., ... & Coulomb L'Herminé, A. (2024). Acinar dysplasia in a full-term newborn with a NKX2. 1 variant. Neonatology, 121(1), 133-136.

Heylen, E., Verstraete, P., Van Aerschot, L., Geeraerts, S. L., Venken, T., Timcheva, K., ... & Kampen, K. R. (2023). Transcription factor NKX2–1 drives serine and glycine synthesis addiction in cancer. British Journal of Cancer, 128(10), 1862-1878.

López, J. M., Jiménez, S., Morona, R., Lozano, D., & Moreno, N. (2022). Analysis of Islet‐1, Nkx2. 1, Pax6, and Orthopedia in the forebrain of the sturgeon Acipenser ruthenus identifies conserved prosomeric characteristics. Journal of Comparative Neurology, 530(5), 834-855.

Teng, F., Zhang, J. X., Chen, Y., Shen, X. D., Su, C., Guo, Y. J., ... & Liu, S. Q. (2021). LncRNA NKX2‐1‐AS1 promotes tumor progression and angiogenesis via upregulation of SERPINE1 expression and activation of the VEGFR‐2 signaling pathway in gastric cancer. Molecular oncology, 15(4), 1234-1255.

Stuart, W. D., Fink-Baldauf, I. M., Tomoshige, K., Guo, M., & Maeda, Y. (2021). CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung. Communications Biology, 4(1), 568.

Valero, M., Viney, T. J., Machold, R., Mederos, S., Zutshi, I., Schuman, B., ... & Buzsáki, G. (2021). Sleep down state-active ID2/Nkx2. 1 interneurons in the neocortex. Nature neuroscience, 24(3), 401-411.

Little, D. R., Lynch, A. M., Yan, Y., Akiyama, H., Kimura, S., & Chen, J. (2021). Differential chromatin binding of the lung lineage transcription factor NKX2-1 resolves opposing murine alveolar cell fates in vivo. Nature communications, 12(1), 2509.

Abecassis, Z. A., Berceau, B. L., Win, P. H., Garcia, D., Xenias, H. S., Cui, Q., ... & Chan, C. S. (2020). Npas1+-Nkx2. 1+ neurons are an integral part of the cortico-pallido-cortical loop. Journal of Neuroscience, 40(4), 743-768.

Murcia-Ramón, R., Company, V., Juárez-Leal, I., Andreu-Cervera, A., Almagro-García, F., Martínez, S., ... & Puelles, E. (2020). Neuronal tangential migration from Nkx2. 1-positive hypothalamus. Brain Structure and Function, 225, 2857-2869.

Orquera, D. P., Tavella, M. B., de Souza, F. S., Nasif, S., Low, M. J., & Rubinstein, M. (2019). The homeodomain transcription factor NKX2. 1 is essential for the early specification of melanocortin neuron identity and activates Pomc expression in the developing hypothalamus. Journal of Neuroscience, 39(21), 4023-4035.

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For research use only. Not intended for any clinical use.

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